1ki1: Difference between revisions
New page: left|200px<br /> <applet load="1ki1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ki1, resolution 2.3Å" /> '''Guanine Nucleotide E... |
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[[Image:1ki1.gif|left|200px]]<br /> | [[Image:1ki1.gif|left|200px]]<br /><applet load="1ki1" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1ki1, resolution 2.3Å" /> | caption="1ki1, resolution 2.3Å" /> | ||
'''Guanine Nucleotide Exchange Region of Intersectin in Complex with Cdc42'''<br /> | '''Guanine Nucleotide Exchange Region of Intersectin in Complex with Cdc42'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1KI1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1KI1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KI1 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: rho gtpase]] | [[Category: rho gtpase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:13:52 2008'' | ||
Revision as of 17:13, 15 February 2008
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Guanine Nucleotide Exchange Region of Intersectin in Complex with Cdc42
OverviewOverview
Activation of Rho-family GTPases involves the removal of bound GDP and the, subsequent loading of GTP, all catalyzed by guanine nucleotide exchange, factors (GEFs) of the Dbl-family. Despite high sequence conservation among, Rho GTPases, Dbl proteins possess a wide spectrum of discriminatory, potentials for Rho-family members. To rationalize this specificity, we, have determined crystal structures of the conserved, catalytic fragments, (Dbl and pleckstrin homology domains) of the exchange factors intersectin, and Dbs in complex with their cognate GTPases, Cdc42 and RhoA, respectively. Structure-based mutagenesis of intersectin and Dbs reveals, the key determinants responsible for promoting exchange activity in Cdc42, Rac1 and RhoA. These findings provide critical insight into the structural, features necessary for the proper pairing of Dbl-exchange factors with Rho, GTPases and now allow for the detailed manipulation of signaling pathways, mediated by these oncoproteins in vivo.
About this StructureAbout this Structure
1KI1 is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for the selective activation of Rho GTPases by Dbl exchange factors., Snyder JT, Worthylake DK, Rossman KL, Betts L, Pruitt WM, Siderovski DP, Der CJ, Sondek J, Nat Struct Biol. 2002 Jun;9(6):468-75. PMID:12006984
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