Cellobiohydrolase

Cellobiohydrolase (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. There are two types of CBH. CBHI cleaves progressively from the reducing end while CBHII cleaves progressively from the nonreducing end of cellulose.

3D structures of cellobiohydrolase3D structures of cellobiohydrolase

Cellobiohydrolase I

1cel – HjCBHI core – Hypocrea jecorina
1cbh, 2cbh – HjCBHI C terminal – NMR
1azh, 1az6, 1azj, 1azk - HjCBHI cellulose-binding domain (mutant) - NMR
2cel, 4cel, 1egn, 1q2b - HjCBHI catalytic domain (mutant)
1gpi - PcCBHI catalytic domain – Phanerochaete chrysosporium
1q9h, 3pfj - TeCBHI catalytic domain – Talaromyces emersonii
2y9l, 2y9n - CBHI catalytic domain – Hypocrea lixii

Cellobiohydrolase I binary complex

3cel - HjCBHI catalytic domain (mutant) + cellobiose
5cel, 1q2e - HjCBHI catalytic domain (mutant) + cellotetraose
6cel - HjCBHI catalytic domain (mutant) + cellopentaose
7cel - HjCBHI catalytic domain (mutant) + glucose
2v3i, 2v3r - HjCBHI catalytic domain + phenanthrenolol
1dy4 - TrCBHI catalytic domain + propranolol – Trichoderma reesei
1h46 - PcCBHI catalytic domain + propranolol
1z3t - PcCBHI catalytic domain + cellobiose
1z3v - PcCBHI catalytic domain + lactose
1z3w - PcCBHI catalytic domain + cellobioimidazole
3pfx - TeCBHI catalytic domain + cellobiose
3pfz - TeCBHI catalytic domain + cellotetraose
3pl3 - TeCBHI catalytic domain + cellopentaose

Cellobiohydrolase II

3cbh – HjCBHII core
1cb2 – HjCBHII catalytic domain (mutant)
1hgw, 1hgy, 1oc6 – HjCBHII catalytic domain (mutant)

Cellobiohydrolase II binary complex

1bvw - HiCBHII catalytic domain + mannose – Humicola insolens
2bvw - HiCBHII catalytic domain + glucose + cellotetraose
1gz1, 1oc5 - HiCBHII catalytic domain (mutant) + cellotetraose
1oc7 - HiCBHII catalytic domain (mutant) + cellopentaose
1ocj - HiCBHII catalytic domain + cellopentaose
1ocb - HiCBHII catalytic domain + fluorescent substrate
1ocn - HiCBHII catalytic domain (mutant) + isofagomine
1qjw - TrCBHII catalytic domain (mutant) + cellotetraose
1qk0 - TrCBHII catalytic domain + polysaccharide
1qk2 - TrCBHII catalytic domain + cellotetraose

Other cellobiohydrolases

1l1y – CtCBH – Clostridium thermocellum
1rq5 - CtCBH (mutant)
1ut9 - CtCBH catalytic domain
1sqj - GeCBH – Geotrichum
3a64, 3vog - CcCBH catalytic domain - Coprinopsis cinerea
3vof, 3voj - CcCBHI catalytic domain (mutant)

Other cellobiohydrolases binary complex

1l2a - CtCBH + glucose
2ebs – GeCBH (mutant) + heptasaccharide
3a9b, 3voh - CcCBHI catalytic domain + cellobiose
3abx, 3voi - CcCBHI catalytic domain + cellotriose