1gcv: Difference between revisions
New page: left|200px<br /> <applet load="1gcv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gcv, resolution 2.0Å" /> '''DEOXY FORM HEMOGLOBI... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1gcv. | [[Image:1gcv.jpg|left|200px]]<br /><applet load="1gcv" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1gcv" size=" | |||
caption="1gcv, resolution 2.0Å" /> | caption="1gcv, resolution 2.0Å" /> | ||
'''DEOXY FORM HEMOGLOBIN FROM MUSTELUS GRISEUS'''<br /> | '''DEOXY FORM HEMOGLOBIN FROM MUSTELUS GRISEUS'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
1GCV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mustelus_griseus Mustelus griseus] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1GCV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mustelus_griseus Mustelus griseus] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCV OCA]. | ||
==Reference== | ==Reference== | ||
| Line 26: | Line 25: | ||
[[Category: hemoglobin]] | [[Category: hemoglobin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:52:37 2008'' | ||
Revision as of 16:52, 15 February 2008
|
DEOXY FORM HEMOGLOBIN FROM MUSTELUS GRISEUS
OverviewOverview
Although many descriptions of adaptive molecular evolution of vertebrate, hemoglobins (Hb) can be found in physiological text books, they are based, mainly on changes of the primary structure and place more emphasis on, conservation than alterations at the functional site. Sequence analysis, alone, however, does not reveal much about the evolution of new functions, in proteins. It was found recently that there are many functionally, important structural differences between human and a ray (Dasyatis akajei), Hb even where sequence is conserved between the two. We have solved the, structures of the deoxy and CO forms of a second cartilaginous fish (a, shark, Mustelus griseus) Hb, and compared it with structures of human Hb, two bony fish Hbs and the ray Hb in order to understand more about how, vertebrate Hbs have functionally evolved by the selection of random amino, acid substitutions. The sequence identity of cartilaginous fish Hb and, human Hb is a little less than 40 %, with many functionally important, amino acid replacements. Wider substitutions than usually considered as, neutral have been accepted in the course of molecular evolution of Hb. As, with the ray Hb, the shark Hb shows functionally important structural, differences from human Hb that involve amino acid substitutions and shifts, of preserved amino acid residues induced by substitutions in other parts, of the molecule. Most importantly, beta E11Val in deoxy human Hb, which, overlaps the ligand binding site and is considered to play a key role in, controlling the oxygen affinity, moves away about 1 A in both the shark, and ray Hbs. Thus adaptive molecular evolution is feasible as a result of, both functionally significant mutations and deviations of preserved amino, acid residues induced by other amino acid substitutions.
About this StructureAbout this Structure
1GCV is a Protein complex structure of sequences from Mustelus griseus with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
The functional similarity and structural diversity of human and cartilaginous fish hemoglobins., Naoi Y, Chong KT, Yoshimatsu K, Miyazaki G, Tame JR, Park SY, Adachi S, Morimoto H, J Mol Biol. 2001 Mar 16;307(1):259-70. PMID:11243818
Page seeded by OCA on Fri Feb 15 15:52:37 2008