1bvv: Difference between revisions
New page: left|200px<br /> <applet load="1bvv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bvv, resolution 1.8Å" /> '''SUGAR RING DISTORTIO... |
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==About this Structure== | ==About this Structure== | ||
1BVV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with XYP and DFX as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BVV OCA]]. | 1BVV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with XYP and DFX as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Structure known Active Sites: ABC and NUC. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BVV OCA]]. | ||
==Reference== | ==Reference== | ||
Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase., Sidhu G, Withers SG, Nguyen NT, McIntosh LP, Ziser L, Brayer GD, Biochemistry. 1999 Apr 27;38(17):5346-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10220321 10220321] | Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase., Sidhu G, Withers SG, Nguyen NT, McIntosh LP, Ziser L, Brayer GD, Biochemistry. 1999 Apr 27;38(17):5346-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10220321 10220321] | ||
[[Category: Bacillus circulans]] | [[Category: Bacillus circulans]] | ||
[[Category: Endo-1,4-beta-xylanase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Brayer, G.D.]] | [[Category: Brayer, G.D.]] | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:02:53 2007'' | ||
Revision as of 13:58, 30 October 2007
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SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FAMILY G/11 XYLANASE
OverviewOverview
The 1.8 A resolution structure of the glycosyl-enzyme intermediate formed, on the retaining beta-1,4-xylanase from Bacillus circulans has been, determined using X-ray crystallographic techniques. The 2-fluoro-xylose, residue bound in the -1 subsite adopts a 2,5B (boat) conformation, allowing atoms C5, O5, C1, and C2 of the sugar to achieve coplanarity as, required at the oxocarbenium ion-like transition states of the, double-displacement catalytic mechanism. Comparison of this structure to, that of a mutant of this same enzyme noncovalently complexed with, xylotetraose [Wakarchuk et al. (1994) Protein Sci. 3, 467-475] reveals a, number of differences beyond the distortion of the sugar moiety. Most, notably, a bifurcated hydrogen bond interaction is formed in the, glycosyl-enzyme ... [(full description)]
About this StructureAbout this Structure
1BVV is a [Single protein] structure of sequence from [Bacillus circulans] with XYP and DFX as [ligands]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Sites: ABC and NUC. Full crystallographic information is available from [OCA].
ReferenceReference
Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase., Sidhu G, Withers SG, Nguyen NT, McIntosh LP, Ziser L, Brayer GD, Biochemistry. 1999 Apr 27;38(17):5346-54. PMID:10220321
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