2by1: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
2BY1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]] with GLC, MG, TRS, TRE and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BY1 OCA]]. | 2BY1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]] with GLC, MG, TRS, TRE and BME as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BY1 OCA]]. | ||
==Reference== | ==Reference== | ||
Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16421442 16421442] | Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16421442 16421442] | ||
[[Category: Alpha-amylase]] | |||
[[Category: Deinococcus radiodurans]] | [[Category: Deinococcus radiodurans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: synchrotron radiation]] | [[Category: synchrotron radiation]] | ||
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Revision as of 13:56, 30 October 2007
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IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION
OverviewOverview
This paper focuses on the radiation-damage effects when applying the same, total X-ray dose to protein crystals at different dose rates. These, experiments have been performed on both a selenomethionated protein and on, bovine trypsin using dose rates that span nearly two orders of magnitude., The results show no clear dose-rate effect on the global indicators of, radiation damage, but a small measurable dose-rate effect could be found, when studying specific radiation damage. It is hypothesized that this, observed dose-rate effect relates to differences in the steady-state, free-radical concentration.
About this StructureAbout this Structure
2BY1 is a [Single protein] structure of sequence from [Deinococcus radiodurans] with GLC, MG, TRS, TRE and BME as [ligands]. Active as [Alpha-amylase], with EC number [3.2.1.1]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442
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