Sandbox 50: Difference between revisions
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In addition to the C11P inhibitor bound in the active site, the crystallized structure showed <scene name='Sandbox_50/Detergents/1'>5 octyl beta-glucoside detergent molecules</scene> (blue) that were stabilized by interactions with C11P and needed for the crystallization. <ref>http://pubs.acs.org/doi/abs/10.1021/bi00009a003</ref> In total, the protein compounds contain 1 covalent inhibitor that can be present in both enantiomers, 1 Ca+ ion, | In addition to the C11P inhibitor bound in the active site, the crystallized structure showed <scene name='Sandbox_50/Detergents/1'>5 octyl beta-glucoside detergent molecules</scene> (blue) that were stabilized by interactions with C11P and needed for the crystallization. <ref>http://pubs.acs.org/doi/abs/10.1021/bi00009a003</ref> In total, the protein compounds contain 1 covalent inhibitor that can be present in both enantiomers, 1 Ca+ ion, <scene name='Sandbox_50/Wate_on_1lpb/1'>293 water molecules</scene>, and 5 detergent molecules. | ||
<scene name='Sandbox_50/Wate_on_1lpb/1'>293 water molecules</scene>, and 5 detergent molecules. | |||
Additional known inhibitors of lipase include: | |||
Tetrahydrolipstatin | |||
Orlistat (Alli) | |||
Monoacylglycerol | |||
Wheat flower (WFL1) | |||
== Lipase Assays == | |||
Current research in Dr. Tims' Chem 412 lab is focused on lipase activity in multi-enzyme dietary supplements. The presence of lipase and its activity has been tested for using a western blot analysis, colorimetric assay, and titrimetric assay. Lipase was present in all three tests on pancreatin samples. Only the titrimetric assay showed lipase activity in the multi-enzyme tablet. The titrimetric and colorimetric assays used an olive oil emulsion as a lipase substrate. Products of the previously described mechanism were detected by a decrease in fluorescent activity of the breakdown products in the colorimetric assay. The titrimetric assay took advantage of the fatty acid products of lipase and how much sodium hydroxide it took to get each reaction to a specific pH. | |||
== References == | == References == | ||