1cf4: Difference between revisions
New page: left|200px<br /> <applet load="1cf4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cf4" /> '''CDC42/ACK GTPASE-BINDING DOMAIN COMPLEX'''<... |
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'''CDC42/ACK GTPASE-BINDING DOMAIN COMPLEX'''<br /> | '''CDC42/ACK GTPASE-BINDING DOMAIN COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1CF4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GNP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1CF4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CF4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:35:28 2008'' | ||
Revision as of 16:35, 15 February 2008
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CDC42/ACK GTPASE-BINDING DOMAIN COMPLEX
OverviewOverview
The proteins Cdc42 and Rac are members of the Rho family of small GTPases, (G proteins), which control signal-transduction pathways that lead to, rearrangements of the cell cytoskeleton, cell differentiation and cell, proliferation. They do so by binding to downstream effector proteins. Some, of these, known as CRIB (for Cdc42/Rac interactive-binding) proteins, bind, to both Cdc42 and Rac, such as the PAK1-3 serine/threonine kinases, whereas others are specific for Cdc42, such as the ACK tyrosine kinases, and the Wiscott-Aldrich-syndrome proteins (WASPs). The effector loop of, Cdc42 and Rac (comprising residues 30-40, also called switch I), is one of, two regions which change conformation on exchange of GDP for GTP. This, region is almost identical in Cdc42 and Racs, indicating that it does not, determine the specificity of these G proteins. Here we report the solution, structure of the complex of Cdc42 with the GTPase-binding domain ofACK., Both proteins undergo significant conformational changes on binding, to, form a new type of G-protein/effector complex. The interaction extends the, beta-sheet in Cdc42 by binding an extended strand from ACK, as seen in, Ras/effector interactions, but it also involves other regions of the G, protein that are important for determining the specificity of effector, binding.
About this StructureAbout this Structure
1CF4 is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the small G protein Cdc42 bound to the GTPase-binding domain of ACK., Mott HR, Owen D, Nietlispach D, Lowe PN, Manser E, Lim L, Laue ED, Nature. 1999 May 27;399(6734):384-8. PMID:10360579
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