2q9e: Difference between revisions
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caption="2q9e, resolution 2.100Å" /> | caption="2q9e, resolution 2.100Å" /> | ||
'''Structure of spin-labeled T4 lysozyme mutant S44R1'''<br /> | '''Structure of spin-labeled T4 lysozyme mutant S44R1'''<br /> | ||
==Overview== | |||
Site-directed spin labeling provides a means for exploring structure and, dynamics in proteins. To interpret the complex EPR spectra that often, arise, it is necessary to characterize the rotamers of the spin-labeled, side chain and the interactions they make with the local environment in, proteins of known structure. For this purpose, crystal structures have, been determined for T4 lysozyme bearing a nitroxide side chain (R1) at the, solvent-exposed helical sites 41 and 44 in the B helix. These sites are of, particular interest in that the corresponding EPR spectra reveal two, dynamic states of R1, one of which is relatively immobilized suggesting, interactions of the nitroxide with the environment. The crystal structures, together with the effect of mutagenesis of nearest neighbors on the motion, of R1 suggest intrahelical interactions of 41R1 with the i + 4 residue and, of 44R1 with the i + 1 residue. Such interactions appear to be specific to, particular rotamers of the R1 side chain. | |||
==About this Structure== | ==About this Structure== | ||
2Q9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ | 2Q9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_d3112 Pseudomonas phage d3112] with <scene name='pdbligand=HED:'>HED</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Sites: <scene name='pdbsite=AC1:Hed+Binding+Site+For+Residue+A+900'>AC1</scene>, <scene name='pdbsite=AC2:Hed+Binding+Site+For+Residue+B+910'>AC2</scene> and <scene name='pdbsite=AC3:Hed+Binding+Site+For+Residue+B+920'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q9E OCA]. | ||
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==Reference== | |||
Structural determinants of nitroxide motion in spin-labeled proteins: Solvent-exposed sites in helix B of T4 lysozyme., Guo Z, Cascio D, Hideg K, Hubbell WL, Protein Sci. 2008 Feb;17(2):228-39. Epub 2007 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18096642 18096642] | |||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Pseudomonas phage d3112]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cascio, D.]] | [[Category: Cascio, D.]] | ||
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[[Category: electron paramagnetic resonance]] | [[Category: electron paramagnetic resonance]] | ||
[[Category: epr]] | [[Category: epr]] | ||
[[Category: hydrolase]] | |||
[[Category: nitroxide]] | [[Category: nitroxide]] | ||
[[Category: spin label]] | [[Category: spin label]] | ||
[[Category: t4 lysozyme]] | [[Category: t4 lysozyme]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 13 08:18:12 2008'' | ||
Revision as of 09:18, 13 February 2008
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Structure of spin-labeled T4 lysozyme mutant S44R1
OverviewOverview
Site-directed spin labeling provides a means for exploring structure and, dynamics in proteins. To interpret the complex EPR spectra that often, arise, it is necessary to characterize the rotamers of the spin-labeled, side chain and the interactions they make with the local environment in, proteins of known structure. For this purpose, crystal structures have, been determined for T4 lysozyme bearing a nitroxide side chain (R1) at the, solvent-exposed helical sites 41 and 44 in the B helix. These sites are of, particular interest in that the corresponding EPR spectra reveal two, dynamic states of R1, one of which is relatively immobilized suggesting, interactions of the nitroxide with the environment. The crystal structures, together with the effect of mutagenesis of nearest neighbors on the motion, of R1 suggest intrahelical interactions of 41R1 with the i + 4 residue and, of 44R1 with the i + 1 residue. Such interactions appear to be specific to, particular rotamers of the R1 side chain.
About this StructureAbout this Structure
2Q9E is a Single protein structure of sequence from Pseudomonas phage d3112 with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
ReferenceReference
Structural determinants of nitroxide motion in spin-labeled proteins: Solvent-exposed sites in helix B of T4 lysozyme., Guo Z, Cascio D, Hideg K, Hubbell WL, Protein Sci. 2008 Feb;17(2):228-39. Epub 2007 Dec 20. PMID:18096642
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