1w1h: Difference between revisions
New page: left|200px<br /> <applet load="1w1h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w1h, resolution 1.45Å" /> '''CRYSTAL STRUCTURE O... |
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==About this Structure== | ==About this Structure== | ||
1W1H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.37 2.7.1.37]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W1H OCA]]. | 1W1H is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_2.7.11.1 Transferred entry: 2.7.11.1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.37 2.7.1.37]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W1H OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Transferred entry: 2.7.11.1]] | |||
[[Category: Aalten, D.M.F.Van.]] | [[Category: Aalten, D.M.F.Van.]] | ||
[[Category: Alessi, D.R.]] | [[Category: Alessi, D.R.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:56:02 2007'' | ||
Revision as of 13:51, 30 October 2007
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CRYSTAL STRUCTURE OF THE PDK1 PLECKSTRIN HOMOLOGY (PH) DOMAIN
OverviewOverview
3-phosphoinositide-dependent protein kinase-1 (PDK1) phosphorylates and, activates many kinases belonging to the AGC subfamily. PDK1 possesses a, C-terminal pleckstrin homology (PH) domain that interacts with, PtdIns(3,4,5)P3/PtdIns(3,4)P2 and with lower affinity to PtdIns(4,5)P2. We, describe the crystal structure of the PDK1 PH domain, in the absence and, presence of PtdIns(3,4,5)P3 and Ins(1,3,4,5)P4. The structures reveal a, 'budded' PH domain fold, possessing an N-terminal extension forming an, integral part of the overall fold, and display an unusually spacious, ligand-binding site. Mutagenesis and lipid-binding studies were used to, define the contribution of residues involved in phosphoinositide binding., Using a novel quantitative binding assay, we found that Ins(1,3,4,5,6)P5, ... [(full description)]
About this StructureAbout this Structure
1W1H is a [Single protein] structure of sequence from [Homo sapiens] with SO4 and GOL as [ligands]. Active as [Transferred entry: 2.7.11.1], with EC number [2.7.1.37]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structural insights into the regulation of PDK1 by phosphoinositides and inositol phosphates., Komander D, Fairservice A, Deak M, Kular GS, Prescott AR, Peter Downes C, Safrany ST, Alessi DR, van Aalten DM, EMBO J. 2004 Oct 13;23(20):3918-28. Epub 2004 Sep 30. PMID:15457207
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Homo sapiens
- Single protein
- Transferred entry: 2.7.11.1
- Aalten, D.M.F.Van.
- Alessi, D.R.
- Deak, M.
- Komander, D.
- GOL
- SO4
- Inositol phosphate
- Pdk1
- Phosphoinositide
- Phosphoinositide dependent protein kinase 1
- Pi3-kinase serine/threonine protein kinase
- Pkb
- Pleckstrin homology domain
- Signal transduction
- Transferase