2r10: Difference between revisions
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caption="2r10, resolution 2.20Å" /> | caption="2r10, resolution 2.20Å" /> | ||
'''Structure of an acetylated Rsc4 tandem bromodomain Histone Chimera'''<br /> | '''Structure of an acetylated Rsc4 tandem bromodomain Histone Chimera'''<br /> | ||
==Overview== | |||
An important issue for chromatin remodeling complexes is how their, bromodomains recognize particular acetylated lysine residues in histones., The Rsc4 subunit of the yeast remodeler RSC contains an essential tandem, bromodomain (TBD) that binds acetylated K14 of histone H3 (H3K14ac). We, report a series of crystal structures that reveal a compact TBD that binds, H3K14ac in the second bromodomain and, remarkably, binds acetylated K25 of, Rsc4 itself in the first bromodomain. Endogenous Rsc4 is acetylated only, at K25, and Gcn5 is identified as necessary and sufficient for Rsc4 K25, acetylation in vivo and in vitro. Rsc4 K25 acetylation inhibits binding to, H3K14ac, and mutation of Rsc4 K25 results in altered growth rates. These, data suggest an autoregulatory mechanism in which Gcn5 performs both the, activating (H3K14ac) and inhibitory (Rsc4 K25ac) modifications, perhaps to, provide temporal regulation. Additional regulatory mechanisms are, indicated as H3S10 phosphorylation inhibits Rsc4 binding to H3K14ac, peptides. | |||
==About this Structure== | ==About this Structure== | ||
2R10 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R10 OCA]. | 2R10 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Edo+Binding+Site+For+Residue+A+788'>AC1</scene> and <scene name='pdbsite=AC2:Edo+Binding+Site+For+Residue+A+789'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R10 OCA]. | ||
==Reference== | |||
Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation., VanDemark AP, Kasten MM, Ferris E, Heroux A, Hill CP, Cairns BR, Mol Cell. 2007 Sep 7;27(5):817-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17803945 17803945] | |||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Heroux, A.]] | [[Category: Heroux, A.]] | ||
[[Category: Hill, C.P.]] | [[Category: Hill, C.P.]] | ||
[[Category: Kasten, M.]] | [[Category: Kasten, M.M.]] | ||
[[Category: VanDemark, A.P.]] | [[Category: VanDemark, A.P.]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:29:02 2008'' | ||