2r02: Difference between revisions
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caption="2r02, resolution 2.60Å" /> | caption="2r02, resolution 2.60Å" /> | ||
'''Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLTSL Late Domain'''<br /> | '''Crystal Structure of ALIX/AIP1 in complex with the HIV-1 YPLTSL Late Domain'''<br /> | ||
==Overview== | |||
Retrovirus budding requires short peptide motifs (late domains) located, within the viral Gag protein that function by recruiting cellular factors., The YPX(n)L late domains of HIV and other lentiviruses recruit the protein, ALIX (also known as AIP1), which also functions in vesicle formation at, the multivesicular body and in the abscission stage of cytokinesis. Here, we report the crystal structures of ALIX in complex with the YPX(n)L late, domains from HIV-1 and EIAV. The two distinct late domains bind at the, same site on the ALIX V domain but adopt different conformations that, allow them to make equivalent contacts. Binding studies and functional, assays verified the importance of key interface residues and revealed that, binding affinities are tuned by context-dependent effects. These results, reveal how YPX(n)L late domains recruit ALIX to facilitate virus budding, and how ALIX can bind YPX(n)L sequences with both n = 1 and n = 3. | |||
==About this Structure== | ==About this Structure== | ||
2R02 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R02 OCA]. | 2R02 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R02 OCA]. | ||
==Reference== | |||
Structural and functional studies of ALIX interactions with YPX(n)L late domains of HIV-1 and EIAV., Zhai Q, Fisher RD, Chung HY, Myszka DG, Sundquist WI, Hill CP, Nat Struct Mol Biol. 2008 Jan;15(1):43-9. Epub 2007 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18066081 18066081] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Fisher,R.D.]] | [[Category: Fisher, R.D.]] | ||
[[Category: Hill, C.P.]] | [[Category: Hill, C.P.]] | ||
[[Category: Zhai,Q.]] | [[Category: Zhai, Q.]] | ||
[[Category: aids]] | [[Category: aids]] | ||
[[Category: apoptosis]] | [[Category: apoptosis]] | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
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