2hwm: Difference between revisions
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caption="2hwm, resolution 1.60Å" /> | caption="2hwm, resolution 1.60Å" /> | ||
'''Crystal structure of Lys12Val/Cys117Val mutant of human acidic fibroblast growth factor at 1.60 angstrom resolution'''<br /> | '''Crystal structure of Lys12Val/Cys117Val mutant of human acidic fibroblast growth factor at 1.60 angstrom resolution'''<br /> | ||
==Overview== | |||
The beta-trefoil protein human fibroblast growth factor-1 (FGF-1) is made, up of a six-stranded antiparallel beta-barrel closed off on one end by, three beta-hairpins, thus exhibiting a 3-fold axis of structural symmetry., The N and C terminus beta-strands hydrogen bond to each other and their, interaction is postulated from both NMR and X-ray structure data to be, important in folding and stability. Specific mutations within the adjacent, N and C terminus beta-strands of FGF-1 are shown to provide a substantial, increase in stability. This increase is largely correlated with an, increased folding rate constant, and with a smaller but significant, decrease in the unfolding rate constant. A series of stabilizing mutations, are subsequently combined and result in a doubling of the DeltaG value of, unfolding. When taken in the context of previous studies of stabilizing, mutations, the results indicate that although FGF-1 is known for generally, poor thermal stability, the beta-trefoil architecture appears capable of, substantial thermal stability. Targeting stabilizing mutations within the, N and C terminus beta-strand interactions of a beta-barrel architecture, may be a generally useful approach to increase protein stability. Such, stabilized mutations of FGF-1 are shown to exhibit significant increases, in effective mitogenic potency, and may prove useful as "second, generation" forms of FGF-1 for application in angiogenic therapy. | |||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
2HWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HWM OCA]. | 2HWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Fmt+Binding+Site+For+Residue+B+141'>AC1</scene>, <scene name='pdbsite=AC2:Fmt+Binding+Site+For+Residue+A+141'>AC2</scene>, <scene name='pdbsite=AC3:Fmt+Binding+Site+For+Residue+A+142'>AC3</scene> and <scene name='pdbsite=AC4:Fmt+Binding+Site+For+Residue+B+142'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HWM OCA]. | ||
==Reference== | |||
Spackling the Crack: Stabilizing Human Fibroblast Growth Factor-1 by Targeting the N and C terminus beta-Strand Interactions., Dubey VK, Lee J, Somasundaram T, Blaber S, Blaber M, J Mol Biol. 2007 Aug 3;371(1):256-68. Epub 2007 May 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17570396 17570396] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: FMT]] | [[Category: FMT]] | ||
[[Category: beta-trefoil]] | [[Category: beta-trefoil]] | ||
[[Category: hormone/growth factor complex]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 6 17:28:13 2008'' | ||