3tf4: Difference between revisions
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[[Image:3tf4.jpg|left|200px]]<br /><applet load="3tf4" size=" | [[Image:3tf4.jpg|left|200px]]<br /><applet load="3tf4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="3tf4, resolution 2.2Å" /> | caption="3tf4, resolution 2.2Å" /> | ||
'''ENDO/EXOCELLULASE:CELLOTRIOSE FROM THERMOMONOSPORA'''<br /> | '''ENDO/EXOCELLULASE:CELLOTRIOSE FROM THERMOMONOSPORA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
3TF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] with GLC and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=AA1:Active Site'>AA1</scene>, <scene name='pdbsite=AB1:Active Site'>AB1</scene>, <scene name='pdbsite=CA1:Ca Binding Site'>CA1</scene>, <scene name='pdbsite=CA2:Ca Binding Site'>CA2</scene>, <scene name='pdbsite=CA3:Ca Binding Site'>CA3</scene> and <scene name='pdbsite=CA4:Ca Binding Site'>CA4</scene>. Full crystallographic information is available from [http:// | 3TF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=AA1:Active+Site'>AA1</scene>, <scene name='pdbsite=AB1:Active+Site'>AB1</scene>, <scene name='pdbsite=CA1:Ca+Binding+Site'>CA1</scene>, <scene name='pdbsite=CA2:Ca+Binding+Site'>CA2</scene>, <scene name='pdbsite=CA3:Ca+Binding+Site'>CA3</scene> and <scene name='pdbsite=CA4:Ca+Binding+Site'>CA4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TF4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: glycosyl hydrolase]] | [[Category: glycosyl hydrolase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:52:35 2008'' | ||
Revision as of 11:52, 3 February 2008
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ENDO/EXOCELLULASE:CELLOTRIOSE FROM THERMOMONOSPORA
OverviewOverview
Cellulase E4 from Thermomonospora fusca is unusual in that it has, characteristics of both exo- and endo-cellulases. Here we report the, crystal structure of a 68K M(r) fragment of E4 (E4-68) at 1.9 A, resolution. E4-68 contains both a family 9 catalytic domain, exhibiting an, (alpha/alpha)6 barrel fold, and a family III cellulose binding domain, having an antiparallel beta-sandwich fold. While neither of these folds is, novel, E4-68 provides the first cellulase structure having interacting, catalytic and cellulose binding domains. The complexes of E4-68 with, cellopentaose, cellotriose and cellobiose reveal conformational changes, associated with ligand binding and allow us to propose a catalytic, mechanism for family 9 enzymes. We also provide evidence that E4 has two, novel characteristics: first it combines exo- and endo-activities and, second, when it functions as an exo-cellulase, it cleaves off, cellotetraose units.
About this StructureAbout this Structure
3TF4 is a Single protein structure of sequence from Thermobifida fusca with and as ligands. Active as Cellulase, with EC number 3.2.1.4 Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.
ReferenceReference
Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:9334746
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