2znc: Difference between revisions
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[[Image:2znc.gif|left|200px]]<br /><applet load="2znc" size=" | [[Image:2znc.gif|left|200px]]<br /><applet load="2znc" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2znc, resolution 2.8Å" /> | caption="2znc, resolution 2.8Å" /> | ||
'''MURINE CARBONIC ANHYDRASE IV'''<br /> | '''MURINE CARBONIC ANHYDRASE IV'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2ZNC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Known structural/functional Site: <scene name='pdbsite=ZN:Zn Binding Site'>ZN</scene>. Full crystallographic information is available from [http:// | 2ZNC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Known structural/functional Site: <scene name='pdbsite=ZN:Zn+Binding+Site'>ZN</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZNC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
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Revision as of 11:51, 3 February 2008
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MURINE CARBONIC ANHYDRASE IV
OverviewOverview
Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to, plasma membrane surfaces by a phosphatidylinositol glycan linkage. We have, determined the 2.8-angstroms resolution crystal structure of a truncated, soluble form of recombinant murine CAIV. We have also determined the, structure of its complex with a drug used for glaucoma therapy, the, sulfonamide inhibitor brinzolamide (Azopt). The overall structure of, murine CAIV is generally similar to that of human CAIV; however, some, local structural differences are found in the active site resulting from, amino acid sequence differences in the "130's segment" and the residue-63, loop (these may affect the nearby catalytic proton shuttle, His-64)., Similar to human CAIV, the C-terminus of murine CAIV is surrounded by a, substantial electropositive surface potential that may stabilize the, interaction with the phospholipid membrane. Binding interactions observed, for brinzolamide rationalize the generally weaker affinity of inhibitors, used in glaucoma therapy toward CAIV compared with CAII.
About this StructureAbout this Structure
2ZNC is a Single protein structure of sequence from Mus musculus with as ligand. Active as Carbonate dehydratase, with EC number 4.2.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: molecular basis of isozyme-drug discrimination., Stams T, Chen Y, Boriack-Sjodin PA, Hurt JD, Liao J, May JA, Dean T, Laipis P, Silverman DN, Christianson DW, Protein Sci. 1998 Mar;7(3):556-63. PMID:9541386
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