Molecular Playground/Human mtRNA pol: Difference between revisions
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Related to the T7 RNA polymerase is the human mitochondrial RNA polymerase <scene name='Molecular_Playground/Human_mtRNA_pol/All/1'>(PDB: 3SPA)</scene>. Recent crystallization of this enzyme by [http://www.ncbi.nlm.nih.gov/pubmed/21947009 Temiakov et al.] has revealed structural similarity between the two polymerases. The <font color =gray> C-terminal </font> | Related to the T7 RNA polymerase is the human mitochondrial RNA polymerase <scene name='Molecular_Playground/Human_mtRNA_pol/All/1'>(PDB: 3SPA)</scene>. Recent crystallization of this enzyme by [http://www.ncbi.nlm.nih.gov/pubmed/21947009 Temiakov et al.] has revealed structural similarity between the two polymerases. The <font color =gray> C-terminal </font> | ||
<scene name='Molecular_Playground/Human_mtRNA_pol/Ctd/2'>domain</scene> contains the active site for RNA synthesis. The <font color=magenta> N-terminal </font><scene name='Molecular_Playground/Human_mtRNA_pol/Ntd/2'>domain</scene>,(NTD) contains the promoter-binding domain (PBD) essential for DNA binding and start site specificity. Key structures of the NTD include the <font color=red> specificity loop </font> <scene name='Molecular_Playground/Human_mtRNA_pol/Specloop/1'> (unresolved)</scene>, <font color=orange> intercalating </font> <scene name='Molecular_Playground/Human_mtRNA_pol/Intercalloop/1'>loop</scene> , and <font color=blue> AT-recognition </font> <scene name='Molecular_Playground/Human_mtRNA_pol/Atloop/2'>loop</scene>. One feature that is unique to the human mtRNA polymerase is the helical <scene name='Molecular_Playground/Human_mtRNA_pol/Ppr/3'>domain</scene> | <scene name='Molecular_Playground/Human_mtRNA_pol/Ctd/2'>domain</scene> contains the active site for RNA synthesis. The <font color=magenta> N-terminal </font><scene name='Molecular_Playground/Human_mtRNA_pol/Ntd/2'>domain</scene>,(NTD) contains the promoter-binding domain (PBD) essential for DNA binding and start site specificity. Key structures of the NTD include the <font color=red> specificity loop </font> <scene name='Molecular_Playground/Human_mtRNA_pol/Specloop/1'> (unresolved)</scene>, <font color=orange> intercalating </font> <scene name='Molecular_Playground/Human_mtRNA_pol/Intercalloop/1'>loop</scene> , and <font color=blue> AT-recognition </font> <scene name='Molecular_Playground/Human_mtRNA_pol/Atloop/2'>loop</scene>. One feature that is unique to the human mtRNA polymerase is the <font color=cyan> helical </font> <scene name='Molecular_Playground/Human_mtRNA_pol/Ppr/3'>domain</scene> before the NTD. This domain is believed to play a role in RNA processing. | ||
One very big difference between the two polymerases is in the form of two transcription factors, TFB2M and TFAM. Human mtRNA polymerase requires these two proteins for binding and melting of promoter DNA. TFAM binds 15-40 base pairs upstream while TFB2M binds more proximal to the transcription start site (1-3). The requirement of these transcription factors coupled with the discrepancy of PBD orientation between T7 and human mtRNA polymerase suggests a role in enzyme remodeling for the transcription factors, namely, TFB2M. By studying the structural changes and differences between the two polymerases, we can better under the energetics that governed transcription. | One very big difference between the two polymerases is in the form of two transcription factors, TFB2M and TFAM. Human mtRNA polymerase requires these two proteins for binding and melting of promoter DNA. TFAM binds 15-40 base pairs upstream while TFB2M binds more proximal to the transcription start site (1-3). The requirement of these transcription factors coupled with the discrepancy of PBD orientation between T7 and human mtRNA polymerase suggests a role in enzyme remodeling for the transcription factors, namely, TFB2M. By studying the structural changes and differences between the two polymerases, we can better under the energetics that governed transcription. | ||
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