2c0y: Difference between revisions
New page: left|200px<br /> <applet load="2c0y" size="450" color="white" frame="true" align="right" spinBox="true" caption="2c0y, resolution 2.10Å" /> '''THE CRYSTAL STRUCTU... |
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==About this Structure== | ==About this Structure== | ||
2C0Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.27 3.4.22.27]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C0Y OCA]]. | 2C0Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]]. Active as [[http://en.wikipedia.org/wiki/Cathepsin_S Cathepsin S]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.27 3.4.22.27]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C0Y OCA]]. | ||
==Reference== | ==Reference== | ||
The crystal structure of a Cys25 -> Ala mutant of human procathepsin S elucidates enzyme-prosequence interactions., Kaulmann G, Palm GJ, Schilling K, Hilgenfeld R, Wiederanders B, Protein Sci. 2006 Nov;15(11):2619-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17075137 17075137] | The crystal structure of a Cys25 -> Ala mutant of human procathepsin S elucidates enzyme-prosequence interactions., Kaulmann G, Palm GJ, Schilling K, Hilgenfeld R, Wiederanders B, Protein Sci. 2006 Nov;15(11):2619-29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17075137 17075137] | ||
[[Category: Cathepsin S]] | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zymogen]] | [[Category: zymogen]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:49:33 2007'' | ||
Revision as of 13:44, 30 October 2007
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THE CRYSTAL STRUCTURE OF A CYS25ALA MUTANT OF HUMAN PROCATHEPSIN S
OverviewOverview
The crystal structure of the active-site mutant Cys25 --> Ala of, glycosylated human procathepsin S is reported. It was determined by, molecular replacement and refined to 2.1 Angstrom resolution, with an, R-factor of 0.198. The overall structure is very similar to other, cathepsin L-like zymogens of the C1A clan. The peptidase unit comprises, two globular domains, and a small third domain is formed by the N-terminal, part of the prosequence. It is anchored to the prosegment binding loop of, the enzyme. Prosegment residues beyond the prodomain dock to the substrate, binding cleft in a nonproductive orientation. Structural comparison with, published data for mature cathepsin S revealed that procathepsin S, residues Phe146, Phe70, and Phe211 adopt different orientations. Being, part of ... [(full description)]
About this StructureAbout this Structure
2C0Y is a [Single protein] structure of sequence from [Homo sapiens]. Active as [Cathepsin S], with EC number [3.4.22.27]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
ReferenceReference
The crystal structure of a Cys25 -> Ala mutant of human procathepsin S elucidates enzyme-prosequence interactions., Kaulmann G, Palm GJ, Schilling K, Hilgenfeld R, Wiederanders B, Protein Sci. 2006 Nov;15(11):2619-29. PMID:17075137
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