Sandbox 27: Difference between revisions
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Studies on the structure of TSH receptor are based upon its binding to an antibody called M22. | Studies on the structure of TSH receptor are based upon its binding to an antibody called M22. | ||
TSH -M22 complex can be studied by crystallography and X-Ray diffraction analysis at 2.55 A resolution. Zinc ions are used as cristallisation additive(there is no specific interaction with the protein). Further information is available from [http://www.ncbi.nlm.nih.gov/pubmed/17542669 Pubmed] <ref> J. Sanders and co Crystal, Structure of the TSH Receptor in Complex with a Thyroid-Stimulating Autoantibody, THYROID Volume 17, Number 5, 2007 PMID:17542669</ref> | TSH - M22 complex can be studied by crystallography and X-Ray diffraction analysis at 2.55 A resolution. Zinc ions are used as cristallisation additive (there is no specific interaction with the protein). Further information is available from [http://www.ncbi.nlm.nih.gov/pubmed/17542669 Pubmed] <ref> J. Sanders and co Crystal, Structure of the TSH Receptor in Complex with a Thyroid-Stimulating Autoantibody, THYROID Volume 17, Number 5, 2007 PMID:17542669 </ref> | ||
M22 is a thyroid stimulating human monoclonal antibody prepared using lymphocytes from a patient with Graves’ disease. It is an antibody to the TSHR. It mimics closely the binding of TSH on its receptor, so it stimulates the receptor and inhibits the binding of TSH to give rise to full activation of receptor mediated signal transduction. | M22 is a thyroid stimulating human monoclonal antibody prepared using lymphocytes from a patient with Graves’ disease. It is an antibody to the TSHR. It mimics closely the binding of TSH on its receptor, so it stimulates the receptor and inhibits the binding of TSH to give rise to full activation of receptor mediated signal transduction. | ||