Sand box 211: Difference between revisions
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It can be used to destroy anything apart from pure closed circular plasmid, so it is useful to increase transfection efficiency and to reduce background in cloning experiments. | It can be used to destroy anything apart from pure closed circular plasmid, so it is useful to increase transfection efficiency and to reduce background in cloning experiments. | ||
T5 5’-exonuclease is also used to remove denatured DNA from alkaline-based plasmid purification for improved cloning procedures. | T5 5’-exonuclease is also used to remove denatured DNA from alkaline-based plasmid purification for improved cloning procedures. | ||
== '''External Resources''' == | |||
* [http://www.rcsb.org/pdb/explore/explore.do?structureId=1UT5 PDB file on 1UT5] | |||
* [http://www.rcsb.org/pdb/explore/explore.do?structureId=1UT8 PDB file on 1UT8] | |||
* [http://www.rcsb.org/pdb/explore/explore.do?structureId=1XO1 PDB file on 1XO1] | |||
== '''References''' == | == '''References''' == | ||