2j7a: Difference between revisions
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[[Image:2j7a.gif|left|200px]]<br /><applet load="2j7a" size=" | [[Image:2j7a.gif|left|200px]]<br /><applet load="2j7a" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2j7a, resolution 2.30Å" /> | caption="2j7a, resolution 2.30Å" /> | ||
'''CRYSTAL STRUCTURE OF CYTOCHROME C NITRITE REDUCTASE NRFHA COMPLEX FROM DESULFOVIBRIO VULGARIS'''<br /> | '''CRYSTAL STRUCTURE OF CYTOCHROME C NITRITE REDUCTASE NRFHA COMPLEX FROM DESULFOVIBRIO VULGARIS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2J7A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with CA, ACT, HEM and LMT as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Act Binding Site For Chain O'>AC1</scene>. Full crystallographic information is available from [http:// | 2J7A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=LMT:'>LMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+O'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7A OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: quinol dehydrogenase]] | [[Category: quinol dehydrogenase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:42:24 2008'' | ||
Revision as of 11:42, 3 February 2008
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CRYSTAL STRUCTURE OF CYTOCHROME C NITRITE REDUCTASE NRFHA COMPLEX FROM DESULFOVIBRIO VULGARIS
OverviewOverview
Oxidation of membrane-bound quinol molecules is a central step in the, respiratory electron transport chains used by biological cells to generate, ATP by oxidative phosphorylation. A novel family of cytochrome c quinol, dehydrogenases that play an important role in bacterial respiratory chains, was recognised in recent years. Here, we describe the first structure of a, cytochrome from this family, NrfH from Desulfovibrio vulgaris, which forms, a stable complex with its electron partner, the cytochrome c nitrite, reductase NrfA. One NrfH molecule interacts with one NrfA dimer in an, asymmetrical manner, forming a large membrane-bound complex with an, overall alpha(4)beta(2) quaternary arrangement. The menaquinol-interacting, NrfH haem is pentacoordinated, bound by a methionine from the CXXCHXM, sequence, with an aspartate residue occupying the distal position. The, NrfH haem that transfers electrons to NrfA has a lysine residue from the, closest NrfA molecule as distal ligand. A likely menaquinol binding site, containing several conserved and essential residues, is identified.
About this StructureAbout this Structure
2J7A is a Protein complex structure of sequences from Desulfovibrio vulgaris with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
X-ray structure of the membrane-bound cytochrome c quinol dehydrogenase NrfH reveals novel haem coordination., Rodrigues ML, Oliveira TF, Pereira IA, Archer M, EMBO J. 2006 Dec 13;25(24):5951-60. Epub 2006 Nov 30. PMID:17139260
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