2iy6: Difference between revisions

Revision as of 11:39, 3 February 2008

1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE FROM THERMUS WITH BOUND CITRATE

OverviewOverview

Delta(1)-pyrroline-5-carboxylate dehydrogenase (P5CDh) plays an important, role in the metabolic pathway from proline to glutamate. It irreversibly, catalyzes the oxidation of glutamate-gamma-semialdehyde, the product of, the non-enzymatic hydrolysis of Delta(1)-pyrroline-5-carboxylate, into, glutamate with the reduction of NAD(+) into NADH. We have confirmed the, P5CDh activity of the Thermus thermophilus protein TT0033 (TtP5CDh), and, determined the crystal structure of the enzyme in the ligand-free form at, 1.4 A resolution. To investigate the structural basis of TtP5CDh function, the TtP5CDh structures with NAD(+), with NADH, and with its product, glutamate were determined at 1.8 A, 1.9 A, and 1.4 A resolution, respectively. The solved structures suggest an overall view of the P5CDh, catalytic mechanism and provide insights into the P5CDh deficiencies in, the case of the human type II hyperprolinemia.

About this StructureAbout this Structure

2IY6 is a Single protein structure of sequence from Thermus thermophilus with , , , and as ligands. Active as 1-pyrroline-5-carboxylate dehydrogenase, with EC number 1.5.1.12 Known structural/functional Site: . Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase., Inagaki E, Ohshima N, Takahashi H, Kuroishi C, Yokoyama S, Tahirov TH, J Mol Biol. 2006 Sep 22;362(3):490-501. Epub 2006 Jul 29. PMID:16934832

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OCA

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-[[Image:2iy6.gif|left|200px]]<br /><applet load="2iy6" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2iy6.gif|left|200px]]<br /><applet load="2iy6" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2iy6, resolution 1.80&Aring;" />
 '''1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE FROM THERMUS WITH BOUND CITRATE'''<br />
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 ==About this Structure==
-IY6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with FLC, NA, CL, MRD and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] Known structural/functional Site: <scene name='pdbsite=AC1:Cl Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IY6 OCA].
+IY6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=FLC:'>FLC</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=MRD:'>MRD</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/1-pyrroline-5-carboxylate_dehydrogenase 1-pyrroline-5-carboxylate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.12 1.5.1.12] Known structural/functional Site: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IY6 OCA].
 ==Reference==
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 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:40:29 2007''
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