Sandbox 212: Difference between revisions
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== The Catalytic Mechanism of Carnitine Acyltransferases== | == The Catalytic Mechanism of Carnitine Acyltransferases== | ||
[[Image:Machanism fatty acid transfer.jpg|thumb| | [[Image:Machanism fatty acid transfer.jpg|thumb|600px|left]] | ||
It is assumed that the whole family of carnitine acyltransferases share the same catalytic mechanism, because certain residues in the catalytic side (histidine343, serine554) are conserved throughout the family. | It is assumed that the whole family of carnitine acyltransferases share the same catalytic mechanism, because certain residues in the catalytic side (histidine343, serine554) are conserved throughout the family. | ||
Histidine 343 is probably the most important residue in catalysis. First, it induces optimal substrate binding by forming a hydrogen bond between the hydrogen of the substrate’s reactive group and its side chain. After all substrate attained the right position, the catalytic histidine residue is ready to extract a proton from either the hydroxyl group of carnitine or the thiol group of CoA. Thus the catalytic histidine residue can be considered as a general base in catalysis.Which proton is extracted depends on the direction of the reaction. Acyl- carnitine is formed by extracting a proton from carnitine, whereas acyl-CoA is formed by extracting a proton from CoA. | Histidine 343 is probably the most important residue in catalysis. First, it induces optimal substrate binding by forming a hydrogen bond between the hydrogen of the substrate’s reactive group and its side chain. After all substrate attained the right position, the catalytic histidine residue is ready to extract a proton from either the hydroxyl group of carnitine or the thiol group of CoA. Thus the catalytic histidine residue can be considered as a general base in catalysis.Which proton is extracted depends on the direction of the reaction. Acyl- carnitine is formed by extracting a proton from carnitine, whereas acyl-CoA is formed by extracting a proton from CoA. | ||