Sandbox 300: Difference between revisions
No edit summary |
No edit summary |
||
| Line 34: | Line 34: | ||
In a normal case of phagocytosis, the bacterium is eliminated during intracellular digestion, thanks to hydrolytic enzymes of phagocyte. At first, the particle is recognized and sticks on the phagocyte. The recognition is possible thanks to phagocyte membrane receptors, which recognize the Fc domain of immunoglobulins. Then the bacteria enter into the phagocyte by a process of endocytosis. The intracellular digestion takes place, and the bacterium is degraded by enzymes. To finish, cell fragments are removed by exocytosis. | In a normal case of phagocytosis, the bacterium is eliminated during intracellular digestion, thanks to hydrolytic enzymes of phagocyte. At first, the particle is recognized and sticks on the phagocyte. The recognition is possible thanks to phagocyte membrane receptors, which recognize the Fc domain of immunoglobulins. Then the bacteria enter into the phagocyte by a process of endocytosis. The intracellular digestion takes place, and the bacterium is degraded by enzymes. To finish, cell fragments are removed by exocytosis. | ||
In the case of S.aureus, in the serum, the interaction between Fc domain and protein A leads to the attachment in a wrong direction of the IgG to the bacterium: then, the recognition is not allowed and the cascade of reactions necessary for phagocytosis does not occur. By stopping this process and through other virulence factors, bacterial colonization is allowed, through the growth and dissemination of bacteria into the organism<ref>http://en.wikipedia.org/wiki/Protein_A?oldid=252478781</ref>. | In the case of S.aureus, in the serum, the interaction between Fc domain and protein A leads to the attachment in a wrong direction of the IgG to the bacterium: then, the recognition is not allowed and the cascade of reactions necessary for phagocytosis does not occur. By stopping this process and through other virulence factors, bacterial colonization is allowed, through the growth and dissemination of bacteria into the organism<ref>http://en.wikipedia.org/wiki/Protein_A?oldid=252478781</ref>. | ||
==Protein A in laboratories== | ==Protein A in laboratories== | ||
===Application in purification of antibodies=== | ===Application in purification of antibodies=== | ||