2enr: Difference between revisions
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[[Image:2enr.jpg|left|200px]]<br /><applet load="2enr" size=" | [[Image:2enr.jpg|left|200px]]<br /><applet load="2enr" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2enr, resolution 2.35Å" /> | caption="2enr, resolution 2.35Å" /> | ||
'''CO-CRYSTALS OF DEMETALLIZED CONCANAVALIN A WITH CADMIUM HAVING A CADMIUM ION BOUND IN BOTH THE S1 SITE AND THE S2 SITE'''<br /> | '''CO-CRYSTALS OF DEMETALLIZED CONCANAVALIN A WITH CADMIUM HAVING A CADMIUM ION BOUND IN BOTH THE S1 SITE AND THE S2 SITE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2ENR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=S1:Transition Metal Binding Site S1 6-Coordinated Octahedra ...'>S1</scene> and <scene name='pdbsite=S2:Ca Binding Site S2 7-Coordinated Distorted Octahedral Co ...'>S2</scene>. Full crystallographic information is available from [http:// | 2ENR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=S1:Transition+Metal+Binding+Site+S1+6-Coordinated+Octahedra+...'>S1</scene> and <scene name='pdbsite=S2:Ca+Binding+Site+S2+7-Coordinated+Distorted+Octahedral+Co+...'>S2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ENR OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: plant lectin]] | [[Category: plant lectin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:37:46 2008'' | ||
Revision as of 11:37, 3 February 2008
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CO-CRYSTALS OF DEMETALLIZED CONCANAVALIN A WITH CADMIUM HAVING A CADMIUM ION BOUND IN BOTH THE S1 SITE AND THE S2 SITE
OverviewOverview
The crystal structures of cadmium/cadmium and zinc/calcium concanavalin A, (con A) at pH 5.0 and pH 6.15, respectively, were determined. The, structure of cadmium/cadmium con A confirms that the secondary, Cd(2+)-binding site S3 is empty at pH 5. The metal-binding sites S1 and S2, are only very slightly affected by the substitution with cadmium. On the, other hand, S1 and S2 and most of the protein surface of zinc/calcium con, A at pH 6.15 differ from other fully metal-bound and carbohydrate-free, structures. Most of these structural differences at the protein surface, are a result of the interplay between metal binding, protonation and, crystal packing. This interplay is expressed by relative rotations and, translations of the con A units in alternative crystal packings and, participation in space-group conversions inside crystals in situ. The, particular crystal packing of zinc/calcium con A creates a novel, zinc-binding site S4. The Zn(2+) ion in S4 ligates two aspartates from one, tetramer and a histidine from a symmetry-related tetramer.
About this StructureAbout this Structure
2ENR is a Single protein structure of sequence from Canavalia ensiformis with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Zinc/calcium- and cadmium/cadmium-substituted concanavalin A: interplay of metal binding, pH and molecular packing., Bouckaert J, Loris R, Wyns L, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1569-76. PMID:11092923
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