2c5k: Difference between revisions
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[[Image:2c5k.gif|left|200px]]<br /><applet load="2c5k" size=" | [[Image:2c5k.gif|left|200px]]<br /><applet load="2c5k" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2c5k, resolution 2.05Å" /> | caption="2c5k, resolution 2.05Å" /> | ||
'''N-TERMINAL DOMAIN OF TLG1 COMPLEXED WITH N-TERMINUS OF VPS51'''<br /> | '''N-TERMINAL DOMAIN OF TLG1 COMPLEXED WITH N-TERMINUS OF VPS51'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2C5K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain T'>AC1</scene>. Full crystallographic information is available from [http:// | 2C5K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+T'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5K OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: vft complex]] | [[Category: vft complex]] | ||
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Revision as of 11:31, 3 February 2008
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N-TERMINAL DOMAIN OF TLG1 COMPLEXED WITH N-TERMINUS OF VPS51
OverviewOverview
Membrane fusion in cells involves the interaction of SNARE proteins on, apposing membranes. Formation of SNARE complexes is preceded by tethering, events, and a number of protein complexes that are thought to mediate this, have been identified. The VFT or GARP complex is required for, endosome-Golgi traffic in yeast. It consists of four subunits, one of, which, Vps51, has been shown to bind specifically to the SNARE Tlg1, which, participates in the same fusion event. We have determined the structure of, the N-terminal domain of Tlg1 bound to a peptide from the N terminus of, Vps51. Binding depends mainly on residues 18-30 of Vps51. These form a, short helix which lies in a conserved groove in the three-helix bundle, formed by Tlg1. Surprisingly, although both Vps51 and Tlg1 are required, for transport to the late Golgi from endosomes, removal of the, Tlg1-binding sequences from Vps51 does not block such traffic in vivo., Thus, this particular interaction cannot be crucial to the process of, vesicle docking or fusion.
About this StructureAbout this Structure
2C5K is a Protein complex structure of sequences from Saccharomyces cerevisiae with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of the interaction between the SNARE Tlg1 and Vps51., Fridmann-Sirkis Y, Kent HM, Lewis MJ, Evans PR, Pelham HR, Traffic. 2006 Feb;7(2):182-90. PMID:16420526
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