2c16: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2c16.gif|left|200px]]<br /><applet load="2c16" size=" | [[Image:2c16.gif|left|200px]]<br /><applet load="2c16" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2c16, resolution 2.02Å" /> | caption="2c16, resolution 2.02Å" /> | ||
'''5-(4-CARBOXY-2-OXO-BUTANE-1-SULFINYL)-4-OXO-PENTANOIC ACID ACID BOUND TO PORPHOBILINOGEN SYNTHASE FROM PSEUDOMONAS AERUGINOSA'''<br /> | '''5-(4-CARBOXY-2-OXO-BUTANE-1-SULFINYL)-4-OXO-PENTANOIC ACID ACID BOUND TO PORPHOBILINOGEN SYNTHASE FROM PSEUDOMONAS AERUGINOSA'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
2C16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with MG, CL, PGE and PEG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] Known structural/functional Site: <scene name='pdbsite=AC1:Peg Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | 2C16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=PGE:'>PGE</scene> and <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] Known structural/functional Site: <scene name='pdbsite=AC1:Peg+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C16 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 33: | Line 33: | ||
[[Category: porphyrin biosynthesis]] | [[Category: porphyrin biosynthesis]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:30:11 2008'' | ||
Revision as of 11:30, 3 February 2008
|
5-(4-CARBOXY-2-OXO-BUTANE-1-SULFINYL)-4-OXO-PENTANOIC ACID ACID BOUND TO PORPHOBILINOGEN SYNTHASE FROM PSEUDOMONAS AERUGINOSA
OverviewOverview
Porphobilinogen synthase catalyzes the first committed step of the, tetrapyrrole biosynthesis pathway. In an aldol-like condensation, two, molecules of 5-aminolevulinic acid (ALA) form the first pyrrole, porphobilinogen. Newly synthesized analogues of a reaction intermediate of, porphobilinogen synthase have been employed in studying the active site, and the catalytic mechanism of this early enzyme of tetrapyrrole, biosynthesis. This study combines structural and kinetic evaluation of the, inhibition potency of these inhibitors. In addition, one of the determined, protein structures provides for the first time structural evidence of a, magnesium ion in the active site. From these results, we can corroborate, an earlier postulated enzymatic mechanism that starts with formation of a, C-C bond, linking C3 of the A-side ALA to C4 of the P-side ALA through an, aldole addition. The obtained data are discussed with respect to the, current literature.
About this StructureAbout this Structure
2C16 is a Single protein structure of sequence from Pseudomonas aeruginosa with , , and as ligands. Active as Porphobilinogen synthase, with EC number 4.2.1.24 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors., Frere F, Nentwich M, Gacond S, Heinz DW, Neier R, Frankenberg-Dinkel N, Biochemistry. 2006 Jul 11;45(27):8243-53. PMID:16819823
Page seeded by OCA on Sun Feb 3 10:30:11 2008