2by1: Difference between revisions
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[[Image:2by1.gif|left|200px]]<br /><applet load="2by1" size=" | [[Image:2by1.gif|left|200px]]<br /><applet load="2by1" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2by1, resolution 1.55Å" /> | caption="2by1, resolution 1.55Å" /> | ||
'''IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION'''<br /> | '''IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2BY1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with GLC, MG, TRS, TRE and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | 2BY1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=TRS:'>TRS</scene>, <scene name='pdbligand=TRE:'>TRE</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BY1 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: synchrotron radiation]] | [[Category: synchrotron radiation]] | ||
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Revision as of 11:29, 3 February 2008
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IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION
OverviewOverview
This paper focuses on the radiation-damage effects when applying the same, total X-ray dose to protein crystals at different dose rates. These, experiments have been performed on both a selenomethionated protein and on, bovine trypsin using dose rates that span nearly two orders of magnitude., The results show no clear dose-rate effect on the global indicators of, radiation damage, but a small measurable dose-rate effect could be found, when studying specific radiation damage. It is hypothesized that this, observed dose-rate effect relates to differences in the steady-state, free-radical concentration.
About this StructureAbout this Structure
2BY1 is a Single protein structure of sequence from Deinococcus radiodurans with , , , and as ligands. Active as Alpha-amylase, with EC number 3.2.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442
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