1e6z: Difference between revisions
New page: left|200px<br /> <applet load="1e6z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e6z, resolution 1.99Å" /> '''CHITINASE B FROM SE... |
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==About this Structure== | ==About this Structure== | ||
1E6Z is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]] with NAG, SO4 and NGO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E6Z OCA]]. | 1E6Z is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]] with NAG, SO4 and NGO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Chitinase Chitinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E6Z OCA]]. | ||
==Reference== | ==Reference== | ||
Structural insights into the catalytic mechanism of a family 18 exo-chitinase., van Aalten DM, Komander D, Synstad B, Gaseidnes S, Peter MG, Eijsink VG, Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):8979-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11481469 11481469] | Structural insights into the catalytic mechanism of a family 18 exo-chitinase., van Aalten DM, Komander D, Synstad B, Gaseidnes S, Peter MG, Eijsink VG, Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):8979-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11481469 11481469] | ||
[[Category: Chitinase]] | |||
[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: chitin degradation]] | [[Category: chitin degradation]] | ||
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Revision as of 13:34, 30 October 2007
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CHITINASE B FROM SERRATIA MARCESCENS WILDTYPE IN COMPLEX WITH CATALYTIC INTERMEDIATE
OverviewOverview
Chitinase B (ChiB) from Serratia marcescens is a family 18 exo-chitinase, whose catalytic domain has a TIM-barrel fold with a tunnel-shaped active, site. We have solved structures of three ChiB complexes that reveal, details of substrate binding, substrate-assisted catalysis, and product, displacement. The structure of an inactive ChiB mutant (E144Q) complexed, with a pentameric substrate (binding in subsites -2 to +3) shows closure, of the "roof" of the active site tunnel. It also shows that the sugar in, the -1 position is distorted to a boat conformation, thus providing, structural evidence in support of a previously proposed catalytic, mechanism. The structures of the active enzyme complexed to allosamidin, (an analogue of a proposed reaction intermediate) and of the active enzyme, ... [(full description)]
About this StructureAbout this Structure
1E6Z is a [Single protein] structure of sequence from [Serratia marcescens] with NAG, SO4 and NGO as [ligands]. Active as [Chitinase], with EC number [3.2.1.14]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structural insights into the catalytic mechanism of a family 18 exo-chitinase., van Aalten DM, Komander D, Synstad B, Gaseidnes S, Peter MG, Eijsink VG, Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):8979-84. PMID:11481469
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