PhoP-PhoQ: Difference between revisions

In this structure, Beryllofluoride (BeF₃^-) is used as a phosphoryl analog to induce the active state conformation. There are numerous bonds that form as a result of BeF₃^- coordination. F₁ of BeF₃^- helps satisfy the octahedral coordination of a present active site Mg²⁺. The conserved active site lysine, Lys101, forms important intramolecular and intermolecular salt bridges, one of which is with F₃ of BeF₃^-. Finally, a conserved "switch residue" Thr79, involved in the activation of all response regulators, forms a H-bond with F₂ of BeF₃^-.