1uzr: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1uzr.jpg|left|200px]]<br /><applet load="1uzr" size=" | [[Image:1uzr.jpg|left|200px]]<br /><applet load="1uzr" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1uzr, resolution 2.20Å" /> | caption="1uzr, resolution 2.20Å" /> | ||
'''CRYSTAL STRUCTURE OF THE CLASS IB RIBONUCLEOTIDE REDUCTASE R2F-2 SUBUNIT FROM MYCOBACTERIUM TUBERCULOSIS'''<br /> | '''CRYSTAL STRUCTURE OF THE CLASS IB RIBONUCLEOTIDE REDUCTASE R2F-2 SUBUNIT FROM MYCOBACTERIUM TUBERCULOSIS'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
1UZR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with FE, CIT and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Known structural/functional Site: <scene name='pdbsite=AC1:Gol Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http:// | 1UZR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=FE:'>FE</scene>, <scene name='pdbligand=CIT:'>CIT</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+C'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZR OCA]. | ||
==Reference== | ==Reference== | ||
| Line 27: | Line 27: | ||
[[Category: tuberculosis]] | [[Category: tuberculosis]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:13:07 2008'' | ||
Revision as of 11:13, 3 February 2008
|
CRYSTAL STRUCTURE OF THE CLASS IB RIBONUCLEOTIDE REDUCTASE R2F-2 SUBUNIT FROM MYCOBACTERIUM TUBERCULOSIS
OverviewOverview
Two nrdF genes of Mycobacterium tuberculosis code for different R2, subunits of the class Ib ribonucleotide reductase (RNR). The proteins are, denoted R2F-1 and R2F-2 having 71% sequence identity. The R2F-2 subunit, forms the biologically active RNR complex with the catalytic R1E-subunit., We present the structure of the reduced R2F-2 subunit to 2.2 A resolution., Comparison of the R2F-2 structure with a model of R2F-1 suggests that the, important differences are located at the C-terminus. We found that within, class Ib, the E-helix close to the iron diiron centre has two preferred, conformations, which cannot be explained by the redox-state of the diiron, centre. In the R2F-2 structure, we also could see a mobility of alphaE in, between the two conformations.
About this StructureAbout this Structure
1UZR is a Single protein structure of sequence from Mycobacterium tuberculosis with , and as ligands. Active as Ribonucleoside-diphosphate reductase, with EC number 1.17.4.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the biologically active form of class Ib ribonucleotide reductase small subunit from Mycobacterium tuberculosis., Uppsten M, Davis J, Rubin H, Uhlin U, FEBS Lett. 2004 Jul 2;569(1-3):117-22. PMID:15225619
Page seeded by OCA on Sun Feb 3 10:13:07 2008