1opy: Difference between revisions
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[[Image:1opy.jpg|left|200px]]<br /><applet load="1opy" size=" | [[Image:1opy.jpg|left|200px]]<br /><applet load="1opy" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1opy, resolution 1.9Å" /> | caption="1opy, resolution 1.9Å" /> | ||
'''KSI'''<br /> | '''KSI'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1OPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] Known structural/functional Site: <scene name='pdbsite=A:Active Site'>A</scene>. Full crystallographic information is available from [http:// | 1OPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] Known structural/functional Site: <scene name='pdbsite=A:Active+Site'>A</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPY OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: pseudomonas putida biotype b]] | [[Category: pseudomonas putida biotype b]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:59:41 2008'' | ||
Revision as of 10:59, 3 February 2008
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KSI
OverviewOverview
The three-dimensional structure of the enzyme 3-oxo-delta5-steroid, isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by, multidimensional heteronuclear magnetic resonance spectroscopy. The two, independently folded monomers pack together by means of extensive, hydrophobic and electrostatic interactions. Each monomer comprises three, alpha helices and a six-strand mixed beta-pleated sheet arranged to form a, deep hydrophobic cavity. Catalytically important residues Tyr14 (general, acid) and Asp38 (general base) are located near the bottom of the cavity, and positioned as expected from mechanistic hypotheses. An unexpected acid, group (Asp99) is also located in the active site adjacent to Tyr14, and, kinetic and binding studies of the Asp99 to Ala mutant demonstrate that, Asp99 contributes to catalysis by stabilizing the intermediate.
About this StructureAbout this Structure
1OPY is a Single protein structure of sequence from Pseudomonas putida. Active as Steroid Delta-isomerase, with EC number 5.3.3.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:9103200
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