1oe9: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1oe9.gif|left|200px]]<br /><applet load="1oe9" size=" | [[Image:1oe9.gif|left|200px]]<br /><applet load="1oe9" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1oe9, resolution 2.05Å" /> | caption="1oe9, resolution 2.05Å" /> | ||
'''CRYSTAL STRUCTURE OF MYOSIN V MOTOR WITH ESSENTIAL LIGHT CHAIN- NUCLEOTIDE-FREE'''<br /> | '''CRYSTAL STRUCTURE OF MYOSIN V MOTOR WITH ESSENTIAL LIGHT CHAIN- NUCLEOTIDE-FREE'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
1OE9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=ATP:So4 Binding Site For Chain A'>ATP</scene>. Full crystallographic information is available from [http:// | 1OE9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=ATP:So4+Binding+Site+For+Chain+A'>ATP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OE9 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 32: | Line 32: | ||
[[Category: unconventional myosin]] | [[Category: unconventional myosin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:57:00 2008'' | ||
Revision as of 10:57, 3 February 2008
|
CRYSTAL STRUCTURE OF MYOSIN V MOTOR WITH ESSENTIAL LIGHT CHAIN- NUCLEOTIDE-FREE
OverviewOverview
The myosin superfamily of molecular motors use ATP hydrolysis and, actin-activated product release to produce directed movement and force., Although this is generally thought to involve movement of a mechanical, lever arm attached to a motor core, the structural details of the, rearrangement in myosin that drive the lever arm motion on actin, attachment are unknown. Motivated by kinetic evidence that the processive, unconventional myosin, myosin V, populates a unique state in the absence, of nucleotide and actin, we obtained a 2.0 A structure of a myosin V, fragment. Here we reveal a conformation of myosin without bound, nucleotide. The nucleotide-binding site has adopted new conformations of, the nucleotide-binding elements that reduce the affinity for the, nucleotide. The major cleft in the molecule has closed, and the lever arm, has assumed a position consistent with that in an actomyosin rigor, complex. These changes have been accomplished by relative movements of the, subdomains of the molecule, and reveal elements of the structural, communication between the actin-binding interface and nucleotide-binding, site of myosin that underlie the mechanism of chemo-mechanical, transduction.
About this StructureAbout this Structure
1OE9 is a Protein complex structure of sequences from Gallus gallus and Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
A structural state of the myosin V motor without bound nucleotide., Coureux PD, Wells AL, Menetrey J, Yengo CM, Morris CA, Sweeney HL, Houdusse A, Nature. 2003 Sep 25;425(6956):419-23. PMID:14508494
Page seeded by OCA on Sun Feb 3 09:57:00 2008