1akd: Difference between revisions
New page: left|200px<br /> <applet load="1akd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1akd, resolution 1.8Å" /> '''CYTOCHROME P450CAM F... |
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==About this Structure== | ==About this Structure== | ||
1AKD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with K, HEM and CAM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AKD OCA]]. | 1AKD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with K, HEM and CAM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]]. Structure known Active Sites: HEM and K. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AKD OCA]]. | ||
==Reference== | ==Reference== | ||
Crystal structure of cytochrome P-450cam complexed with the (1S)-camphor enantiomer., Schlichting I, Jung C, Schulze H, FEBS Lett. 1997 Oct 6;415(3):253-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9357977 9357977] | Crystal structure of cytochrome P-450cam complexed with the (1S)-camphor enantiomer., Schlichting I, Jung C, Schulze H, FEBS Lett. 1997 Oct 6;415(3):253-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9357977 9357977] | ||
[[Category: Camphor 5-monooxygenase]] | |||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxygenase]] | [[Category: oxygenase]] | ||
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Revision as of 13:28, 30 October 2007
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CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR
OverviewOverview
The crystal structure of cytochrome P-450cam complexed with the enantiomer, (1S)-camphor has been solved to 1.8 angstroms resolution and compared with, the structure of the (1R)-camphor P-450cam complex. The overall protein, structure is the same for both enantiomer complexes. However, the, orientation of the substrates in the heme pocket differs. In contrast to, (1R)-camphor, the (1S)-enantiomer binds in at least two orientations. The, major binding mode of (1S)-camphor resembles the one of the, (1R)-enantiomer in that there is a hydrogen bond between Tyr-96 and the, quinone group of camphor, and the 10-methyl group points towards the, I-helix. The binding differs in that C-5 is not at a position suitable for, hydroxylation. In the other orientation (1S)-camphor is not hydrogen, bonded, ... [(full description)]
About this StructureAbout this Structure
1AKD is a [Single protein] structure of sequence from [Pseudomonas putida] with K, HEM and CAM as [ligands]. Active as [Camphor 5-monooxygenase], with EC number [1.14.15.1]. Structure known Active Sites: HEM and K. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of cytochrome P-450cam complexed with the (1S)-camphor enantiomer., Schlichting I, Jung C, Schulze H, FEBS Lett. 1997 Oct 6;415(3):253-7. PMID:9357977
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