1hi5: Difference between revisions
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[[Image:1hi5.gif|left|200px]]<br /><applet load="1hi5" size=" | [[Image:1hi5.gif|left|200px]]<br /><applet load="1hi5" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1hi5, resolution 1.80Å" /> | caption="1hi5, resolution 1.80Å" /> | ||
'''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX'''<br /> | '''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX'''<br /> | ||
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==Disease== | ==Disease== | ||
Known | Known disease associated with this structure: High density lipoprotein cholesterol level QTL 7 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131240 131240]] | ||
==About this Structure== | ==About this Structure== | ||
1HI5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Known structural/functional Site: <scene name='pdbsite=ADP:Adp Binding Site For Chain A'>ADP</scene>. Full crystallographic information is available from [http:// | 1HI5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Known structural/functional Site: <scene name='pdbsite=ADP:Adp+Binding+Site+For+Chain+A'>ADP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI5 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: rnase-2]] | [[Category: rnase-2]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:49:22 2008'' | ||
Revision as of 10:49, 3 February 2008
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EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX
OverviewOverview
Eosinophil-derived neurotoxin (EDN), a basic ribonuclease found in the, large specific granules of eosinophils, belongs to the pancreatic RNase A, family. Although its physiological function is still unclear, it has been, shown that EDN is a neurotoxin capable of inducing the Gordon phenomenon, in rabbits. EDN is also a potent helminthotoxin and can mediate antiviral, activity of eosinophils against isolated virions of the respiratory, syncytial virus. EDN is a catalytically efficient RNase sharing similar, substrate specificity with pancreatic RNase A with its ribonucleolytic, activity being absolutely essential for its neurotoxic, helminthotoxic, and antiviral activities. The crystal structure of recombinant human EDN, in the unliganded form has been determined previously (Mosimann, S. C., Newton, D. L., Youle, R. J., and James, M. N. G. (1996) J. Mol. Biol. 260, 540-552). We have now determined high resolution (1.8 A) crystal, structures for EDN in complex with adenosine-3',5'-diphosphate, (3',5'-ADP), adenosine-2',5'-di-phosphate (2',5'-ADP), adenosine-5'-diphosphate (5'-ADP) as well as for a native structure in the, presence of sulfate refined at 1.6 A. The inhibition constant of these, mononucleotides for EDN has been determined. The structures present the, first detailed picture of differences between EDN and RNase A in substrate, recognition at the ribonucleolytic active site. They also provide a, starting point for the design of tight-binding inhibitors, which may be, used to restrain the RNase activity of EDN.
DiseaseDisease
Known disease associated with this structure: High density lipoprotein cholesterol level QTL 7 OMIM:[131240]
About this StructureAbout this Structure
1HI5 is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors., Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR, J Biol Chem. 2001 May 4;276(18):15009-17. Epub 2001 Jan 11. PMID:11154698
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