1hfk: Difference between revisions
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[[Image:1hfk.gif|left|200px]]<br /><applet load="1hfk" size=" | [[Image:1hfk.gif|left|200px]]<br /><applet load="1hfk" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1hfk, resolution 2.17Å" /> | caption="1hfk, resolution 2.17Å" /> | ||
'''ASPARAGINASE FROM ERWINIA CHRYSANTHEMI, HEXAGONAL FORM WITH WEAK SULFATE'''<br /> | '''ASPARAGINASE FROM ERWINIA CHRYSANTHEMI, HEXAGONAL FORM WITH WEAK SULFATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1HFK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Known structural/functional Sites: <scene name='pdbsite=AS1:Active Site Chain A'>AS1</scene> and <scene name='pdbsite=AS2:Active Site Chain C'>AS2</scene>. Full crystallographic information is available from [http:// | 1HFK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] Known structural/functional Sites: <scene name='pdbsite=AS1:Active+Site+Chain+A'>AS1</scene> and <scene name='pdbsite=AS2:Active+Site+Chain+C'>AS2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HFK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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Revision as of 10:49, 3 February 2008
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ASPARAGINASE FROM ERWINIA CHRYSANTHEMI, HEXAGONAL FORM WITH WEAK SULFATE
OverviewOverview
Quasi-enantiomorphic crystals of the Y25F mutant of Escherichia coli, L-asparaginase and of the native Erwinia chrysanthemi L-asparaginase were, obtained in the hexagonal space groups P6(5)22 and P6(1)22, respectively., The structures of these highly homologous enzymes were solved by molecular, replacement and were refined with data extending to 2.2-2.5 A. These, structures were compared with each other, as well as with other, L-asparaginase structures previously observed with different crystal, packing. It is concluded that the observed phenomenon, which is rare, was, most likely to have arisen by chance.
About this StructureAbout this Structure
1HFK is a Single protein structure of sequence from Erwinia chrysanthemi with as ligand. Active as Asparaginase, with EC number 3.5.1.1 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Structures of two highly homologous bacterial L-asparaginases: a case of enantiomorphic space groups., Jaskolski M, Kozak M, Lubkowski J, Palm G, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):369-77. PMID:11223513
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