1h5d: Difference between revisions
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[[Image:1h5d.jpg|left|200px]]<br /><applet load="1h5d" size=" | [[Image:1h5d.jpg|left|200px]]<br /><applet load="1h5d" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1h5d, resolution 1.60Å" /> | caption="1h5d, resolution 1.60Å" /> | ||
'''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (0-11% DOSE)'''<br /> | '''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (0-11% DOSE)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1H5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana] with ACT, CA and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] Known structural/functional Site: <scene name='pdbsite=HEM:Hem Binding Site For Chain A'>HEM</scene>. Full crystallographic information is available from [http:// | 1H5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] Known structural/functional Site: <scene name='pdbsite=HEM:Hem+Binding+Site+For+Chain+A'>HEM</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5D OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: x-ray induced reduction]] | [[Category: x-ray induced reduction]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:46:36 2008'' | ||
Revision as of 10:46, 3 February 2008
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X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (0-11% DOSE)
OverviewOverview
A molecular description of oxygen and peroxide activation in biological, systems is difficult, because electrons liberated during X-ray data, collection reduce the active centres of redox enzymes catalysing these, reactions. Here we describe an effective strategy to obtain crystal, structures for high-valency redox intermediates and present a, three-dimensional movie of the X-ray-driven catalytic reduction of a bound, dioxygen species in horseradish peroxidase (HRP). We also describe, separate experiments in which high-resolution structures could be obtained, for all five oxidation states of HRP, showing such structures with, preserved redox states for the first time.
About this StructureAbout this Structure
1H5D is a Single protein structure of sequence from Armoracia rusticana with , and as ligands. Active as Peroxidase, with EC number 1.11.1.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:12024218
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