1h50: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1h50.jpg|left|200px]]<br /><applet load="1h50" size=" | [[Image:1h50.jpg|left|200px]]<br /><applet load="1h50" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1h50, resolution 1.50Å" /> | caption="1h50, resolution 1.50Å" /> | ||
'''STUCTURE OF PENTAERYTHRITOL TETRANIRATE REDUCTASE AND COMPLEXES'''<br /> | '''STUCTURE OF PENTAERYTHRITOL TETRANIRATE REDUCTASE AND COMPLEXES'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
1H50 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae] with ACT and FMN as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=ACT:Acetate Is Bound In The Active Site'>ACT</scene> and <scene name='pdbsite=FMN:Fmn Binding Site For Chain A'>FMN</scene>. Full crystallographic information is available from [http:// | 1H50 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=ACT:Acetate+Is+Bound+In+The+Active+Site'>ACT</scene> and <scene name='pdbsite=FMN:Fmn+Binding+Site+For+Chain+A'>FMN</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H50 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 21: | Line 21: | ||
[[Category: steroid binding]] | [[Category: steroid binding]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:46:26 2008'' | ||
Revision as of 10:46, 3 February 2008
|
STUCTURE OF PENTAERYTHRITOL TETRANIRATE REDUCTASE AND COMPLEXES
OverviewOverview
Pentaerythritol tetranitrate reductase (PETN reductase) degrades high, explosive molecules including nitrate esters, nitroaromatics and cyclic, triazine compounds. The enzyme also binds a variety of cyclic enones, including steroids; some steroids act as substrates whilst others are, inhibitors. Understanding the basis of reactivity with cyclic enones, requires structural information for the enzyme and key complexes formed, with steroid substrates and inhibitors. The crystal structure of oxidised, and reduced PETN reductase at 1.5 A resolution establishes a close, structural similarity to the beta/alpha-barrel flavoenzyme, old yellow, enzyme. In complexes of oxidised PETN reductase with progesterone (an, inhibitor), 1,4-androstadiene-3,17-dione and prednisone (both substrates), the steroids are stacked over the si-face of the flavin in an orientation, different from that reported for old yellow enzyme. The specifically, reducible 1,2 unsaturated bonds in 1,4-androstadiene-3,17-dione and, prednisone are not optimally aligned with the flavin N5 in oxidised enzyme, complexes. These structures suggest either relative "flipping" or shifting, of the steroid with respect to the flavin when bound in different redox, forms of the enzyme. Deuterium transfer from nicotinamide coenzyme to, 1,4-androstadiene-3,17-dione via the enzyme bound FMN indicates 1alpha, addition at the steroid C2 atom. These studies rule out lateral motion of, the steroid and indicate that the steroid orientation is "flipped" in, different redox states of the enzyme.
About this StructureAbout this Structure
1H50 is a Single protein structure of sequence from Enterobacter cloacae with and as ligands. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme., Barna TM, Khan H, Bruce NC, Barsukov I, Scrutton NS, Moody PC, J Mol Biol. 2001 Jul 6;310(2):433-47. PMID:11428899
Page seeded by OCA on Sun Feb 3 09:46:26 2008