Shiga toxin: Difference between revisions
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<Structure load='1R4Q' size='500' frame='true' align='right' caption='Shiga Toxin Type | <Structure load='1R4Q' size='500' frame='true' align='right' caption='Shiga Toxin Type 2 (Stx2)'/> | ||
==Introduction== | ==Introduction== | ||
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Shiga Toxin acts as an N-glycosidase, removing an adenine from the 60S ribosomal rRNA of a target cell leading to reduced protein synthesis.<ref name=Di>PMID: 21184769</ref> The B subunit is necessary for binding to eukaryotic cell surface, where it is then endocytosed and proteolytically cleaved into two active A subunits and a B subunit. On the A subunit <scene name='Shiga_toxin/Active_site/1'>Tyr77, Tyr114, Glu167, Arg170, and Trp203</scene> are all essential in glycosidic activity.<ref name=Di>PMID: 21184769</ref> | Shiga Toxin acts as an N-glycosidase, removing an adenine from the 60S ribosomal rRNA of a target cell leading to reduced protein synthesis.<ref name=Di>PMID: 21184769</ref> The B subunit is necessary for binding to eukaryotic cell surface, where it is then endocytosed and proteolytically cleaved into two active A subunits and a B subunit. On the A subunit <scene name='Shiga_toxin/Active_site/1'>Tyr77, Tyr114, Glu167, Arg170, and Trp203</scene> are all essential in glycosidic activity.<ref name=Di>PMID: 21184769</ref> | ||
==3D structures== | |||
[[1dm0]] - Stx1,br /> | |||
[[1r4q]] - Stx2<br /> | |||
[[1r4p]] - Stx2 bound to ligand<br /> | |||
[[2ga4]] - Stx2 with adenine<br /> | |||
'''Mutants''' | |||
[[1c48]] - Shiga-like toxin B subunit<br /> | |||
[[1cqf]] - Shiga-like toxin B subunit bound to trisaccharide<br /> | |||
[[1bos]] - Shiga-like toxin bound to receptor<br /> | |||
==References== | ==References== | ||
{{Reflist}} | {{Reflist}} | ||