1grr: Difference between revisions
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[[Image:1grr.jpg|left|200px]]<br /><applet load="1grr" size=" | [[Image:1grr.jpg|left|200px]]<br /><applet load="1grr" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1grr, resolution 2.90Å" /> | caption="1grr, resolution 2.90Å" /> | ||
'''CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH 2-NAC-CHLORAMPHENICOL FROM STREPTOMYCES VENEZUELAE'''<br /> | '''CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH 2-NAC-CHLORAMPHENICOL FROM STREPTOMYCES VENEZUELAE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1GRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae] with SO4 and CLC as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=SO4:Clc Binding Site For Chain A'>SO4</scene>. Full crystallographic information is available from [http:// | 1GRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CLC:'>CLC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=SO4:Clc+Binding+Site+For+Chain+A'>SO4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GRR OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
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Revision as of 10:42, 3 February 2008
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CHLORAMPHENICOL PHOSPHOTRANSFERASE IN COMPLEX WITH 2-NAC-CHLORAMPHENICOL FROM STREPTOMYCES VENEZUELAE
OverviewOverview
Streptomyces venezuelae synthesizes chloramphenicol (Cm), an inhibitor of, ribosomal peptidyl transferase activity, thereby inhibiting bacterial, growth. The producer escapes autoinhibition by its own secondary, metabolite through phosphorylation of Cm by chloramphenicol, phosphotransferase (CPT). In addition to active site binding, CPT binds, its product 3-phosphoryl-Cm, in an alternate product binding site. To, address the mechanisms of Cm tolerance of the producer, the crystal, structures of CPT were determined in complex with either the, nonchlorinated Cm (2-N-Ac-Cm) at 3.1 A resolution or the antibiotic's, immediate precursor, the p-amino analog p-NH(2)-Cm, at 2.9 A resolution., Surprisingly, p-NH(2)-Cm binds CPT in a novel fashion. Additionally, neither 2-N-Ac-Cm nor p-NH(2)-Cm binds to the secondary product binding, site.
About this StructureAbout this Structure
1GRR is a Single protein structure of sequence from Streptomyces venezuelae with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for chloramphenicol tolerance in Streptomyces venezuelae by chloramphenicol phosphotransferase activity., Izard T, Protein Sci. 2001 Aug;10(8):1508-13. PMID:11468347
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