1fie: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
No edit summary
No edit summary
Line 1: Line 1:
[[Image:1fie.gif|left|200px]]<br /><applet load="1fie" size="450" color="white" frame="true" align="right" spinBox="true"  
[[Image:1fie.gif|left|200px]]<br /><applet load="1fie" size="350" color="white" frame="true" align="right" spinBox="true"  
caption="1fie, resolution 2.5&Aring;" />
caption="1fie, resolution 2.5&Aring;" />
'''RECOMBINANT HUMAN COAGULATION FACTOR XIII'''<br />
'''RECOMBINANT HUMAN COAGULATION FACTOR XIII'''<br />
Line 10: Line 10:


==About this Structure==
==About this Structure==
1FIE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] Known structural/functional Sites: <scene name='pdbsite=CAT:Active Site, Catalytic Triad'>CAT</scene> and <scene name='pdbsite=CBT:Active Site, Catalytic Triad'>CBT</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FIE OCA].  
1FIE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] Known structural/functional Sites: <scene name='pdbsite=CAT:Active+Site,+Catalytic+Triad'>CAT</scene> and <scene name='pdbsite=CBT:Active+Site,+Catalytic+Triad'>CBT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FIE OCA].  


==Reference==
==Reference==
Line 23: Line 23:
[[Category: transferase]]
[[Category: transferase]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 15:16:11 2007''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:39:52 2008''

Revision as of 10:39, 3 February 2008

File:1fie.gif


1fie, resolution 2.5Å

Drag the structure with the mouse to rotate

RECOMBINANT HUMAN COAGULATION FACTOR XIII

OverviewOverview

The three-dimensional structure of the recombinant human factor XIII a2, dimer after cleavage by thrombin has been determined by X-ray, crystallography. Factor XIII zymogen was treated with bovine, alpha-thrombin in the presence of 3 mM CaCl2, and the cleaved protein was, crystallized from Tris buffered at pH 6.5 using ethanol as the, precipitating agent. Refinement of the molecular model of thrombin-cleaved, factor XIII against diffraction data from 10.0 to 2.5 A resolution has, been carried out to give a crystallographic R factor of 18.2%. The, structure of thrombin-cleaved factor XIII is remarkably similar to that of, the zymogen: there are no large conformational changes in the protein and, the 37 residue amino terminus activation peptide remains associated with, the rest of the molecule. This work shows that the activation peptide, upon thrombin cleavage, has the same conformation and occupies the same, position with respect to the rest of the molecule as it does in the, zymogen structure.

DiseaseDisease

Known disease associated with this structure: Factor XIIIA deficiency OMIM:[134570]

About this StructureAbout this Structure

1FIE is a Single protein structure of sequence from Homo sapiens. Active as Protein-glutamine gamma-glutamyltransferase, with EC number 2.3.2.13 Known structural/functional Sites: and . Full crystallographic information is available from OCA.

ReferenceReference

Structural evidence that the activation peptide is not released upon thrombin cleavage of factor XIII., Yee VC, Pedersen LC, Bishop PD, Stenkamp RE, Teller DC, Thromb Res. 1995 Jun 1;78(5):389-97. PMID:7660355

Page seeded by OCA on Sun Feb 3 09:39:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA