Exonuclease: Difference between revisions
Michal Harel (talk | contribs) New page: {{STRUCTURE_1w36| PDB=1w36 | SIZE=400| SCENE= |right|CAPTION=E. coli exonuclease V complex with DNA hairpin, 1w36 }} '''Exonucleases''' (ExN) cleave nucleotides at the end of a pol... |
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{{STRUCTURE_1w36| PDB=1w36 | SIZE=400| SCENE= |right|CAPTION=E. coli exonuclease V complex with DNA hairpin, [[1w36]] }} | {{STRUCTURE_1w36| PDB=1w36 | SIZE=400| SCENE= |right|CAPTION=E. coli exonuclease V complex with DNA hairpin, [[1w36]] }} | ||
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===ExN-I=== | ===ExN-I=== | ||
[[1fxx]], [[3c95]] – EcExN-I – Escherichia coli<br /> | [[1fxx]], [[3c95]] – EcExN-I – ''Escherichia coli''<br /> | ||
[[2qxf]] - EcExN-I + TMP<br /> | [[2qxf]] - EcExN-I + TMP<br /> | ||
[[3hl8]], [[3hp9]] - EcExN-I + inhibitor<br /> | [[3hl8]], [[3hp9]] - EcExN-I + inhibitor<br /> | ||
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===ExN-III=== | ===ExN-III=== | ||
[[2voa]] – ExN-III (mutant) + DNA – Archaeoglobus fulgidus<br /> | [[2voa]] – ExN-III (mutant) + DNA – ''Archaeoglobus fulgidus''<br /> | ||
[[3g1k]] - MtExN-III – Methanothermobacter thermaautotrophicus<br /> | [[3g1k]] - MtExN-III – ''Methanothermobacter thermaautotrophicus''<br /> | ||
[[3g3c]], [[3g4t]] - MtExN-III + DNA<br /> | [[3g3c]], [[3g4t]] - MtExN-III + DNA<br /> | ||
[[3fzi]], [[3g8v]], [[3g91]] - MtExN-III (mutant) <br /> | [[3fzi]], [[3g8v]], [[3g91]] - MtExN-III (mutant) <br /> | ||
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===ExN-IV=== | ===ExN-IV=== | ||
[[2jc4]] – ExN-IV – Nisseria meningitides | [[2jc4]] – ExN-IV – ''Nisseria meningitides'' | ||
===ExN-V=== | ===ExN-V=== | ||
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[[1w36]], [[3k70]] – EcExN-V α+β+γ + DNA hairpin<br /> | [[1w36]], [[3k70]] – EcExN-V α+β+γ + DNA hairpin<br /> | ||
[[3e1s]] - EcExN-V RECD subunit<br /> | [[3e1s]] - EcExN-V RECD subunit<br /> | ||
[[3gp8]] - DrExN-V RECD subunit + DNA – Deinococcus radiodurans<br /> | [[3gp8]] - DrExN-V RECD subunit + DNA – ''Deinococcus radiodurans''<br /> | ||
[[3gpl]] - DrExN-V RECD subunit + DNA + ADPNP | [[3gpl]] - DrExN-V RECD subunit + DNA + ADPNP | ||
===ExN-VII=== | ===ExN-VII=== | ||
[[1vp7]] – ExN-VII small subunit – Bordetella pertussis | [[1vp7]] – ExN-VII small subunit – ''Bordetella pertussis'' | ||
===ExN-VIII=== | ===ExN-VIII=== | ||
Revision as of 14:52, 21 September 2011
Exonucleases (ExN) cleave nucleotides at the end of a polynucleotide chain. In E. coli there are ExN I to VIII. ExN-I cleaves single-stranded DNA; ExN-II is associated with DNA polymerase; ExN-III cleaves double-stranded DNA; ExN-IV cleavage results in nucleoside monophosphate; ExN-V cleaves linear double-stranded DNA; ExN-VIII requires a free 5’ OH group for reacting. ExN were found in human and yeast as well.
3D Structures of exonuclease3D Structures of exonuclease
ExN-IExN-I
1fxx, 3c95 – EcExN-I – Escherichia coli
2qxf - EcExN-I + TMP
3hl8, 3hp9 - EcExN-I + inhibitor
3c94 - EcExN-I + DNA-binding C terminal peptide
ExN-IIIExN-III
2voa – ExN-III (mutant) + DNA – Archaeoglobus fulgidus
3g1k - MtExN-III – Methanothermobacter thermaautotrophicus
3g3c, 3g4t - MtExN-III + DNA
3fzi, 3g8v, 3g91 - MtExN-III (mutant)
3g00, 3g0r, 3g2c, 3g2d, 3g38, 3g3y, 3ga6 - MtExN-III (mutant) + DNA
3g0a - MtExN-III (mutant) + Mn
ExN-IVExN-IV
2jc4 – ExN-IV – Nisseria meningitides
ExN-VExN-V
1w36, 3k70 – EcExN-V α+β+γ + DNA hairpin
3e1s - EcExN-V RECD subunit
3gp8 - DrExN-V RECD subunit + DNA – Deinococcus radiodurans
3gpl - DrExN-V RECD subunit + DNA + ADPNP
ExN-VIIExN-VII
1vp7 – ExN-VII small subunit – Bordetella pertussis
ExN-VIIIExN-VIII
3h4r - EcExN-VIII C terminal (mutant)