1e5t: Difference between revisions
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[[Image:1e5t.jpg|left|200px]]<br /><applet load="1e5t" size=" | [[Image:1e5t.jpg|left|200px]]<br /><applet load="1e5t" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1e5t, resolution 1.7Å" /> | caption="1e5t, resolution 1.7Å" /> | ||
'''PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT'''<br /> | '''PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1E5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] Known structural/functional Sites: <scene name='pdbsite=AS:Active Site, Catalytic Triad'>AS</scene> and <scene name='pdbsite=SS:Disulfide Engineered Between Cys78 And Gln397cys'>SS</scene>. Full crystallographic information is available from [http:// | 1E5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Prolyl_oligopeptidase Prolyl oligopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.26 3.4.21.26] Known structural/functional Sites: <scene name='pdbsite=AS:Active+Site,+Catalytic+Triad'>AS</scene> and <scene name='pdbsite=SS:Disulfide+Engineered+Between+Cys78+And+Gln397cys'>SS</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5T OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: prolyl oligopeptidase]] | [[Category: prolyl oligopeptidase]] | ||
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Revision as of 10:37, 3 February 2008
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PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT
OverviewOverview
Proteases have a variety of strategies for selecting substrates in order, to prevent uncontrolled protein degradation. A recent crystal structure, determination of prolyl oligopeptidase has suggested a way for substrate, selection involving an unclosed seven-bladed beta-propeller domain. We, have engineered a disulfide bond between the first and seventh blades of, the propeller, which resulted in the loss of enzymatic activity. These, results provided direct evidence for a novel strategy of regulation in, which oscillating propeller blades act as a gating filter during, catalysis, letting small peptide substrates into the active site while, excluding large proteins to prevent accidental proteolysis.
About this StructureAbout this Structure
1E5T is a Single protein structure of sequence from Sus scrofa with as ligand. Active as Prolyl oligopeptidase, with EC number 3.4.21.26 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Catalysis of serine oligopeptidases is controlled by a gating filter mechanism., Fulop V, Szeltner Z, Polgar L, EMBO Rep. 2000 Sep;1(3):277-81. PMID:11256612
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