The Bacterial Flagellar Hook: Difference between revisions
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===FlgE31 2004=== | ===FlgE31 2004=== | ||
<applet load='1wlg' size='400' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/1wlg_1_mmol/3' caption= | <applet load='1wlg' size='400' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/1wlg_1_mmol/3' caption=Salmonella typhimurium flagellar hook protein FLGE [[1wlg]]/> | ||
In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length 402-residue protein). Removal of the ends of the full-length chain (domain D0, see below) was required in order to coax the protein to crystallize, instead of forming filaments. | In 2004, Samatey ''et al.'' solved the structure of the mid-portion of the wild-type FlgE of ''Salmonella typhimurium'' by [[X-ray crystallography]] at a [[Resolution|resolution]] of 1.8 Å ([[1wlg]])<ref name="hook1">PMID: 15510139</ref>. The fragment successfully crystallized, designated FlgE31, consisted of amino acids 71-369, of which 71-363 were resolved (72% of the full-length 402-residue protein). Removal of the ends of the full-length chain (domain D0, see below) was required in order to coax the protein to crystallize, instead of forming filaments. | ||