Telomerase Reverse Transcriptase: Difference between revisions
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== '''Experimental fragment with TEN''' == | == '''Experimental fragment with TEN''' == | ||
<applet load='2b2a' size='400' frame='true' align='right' caption='Tetrahymena thermophila telomerase reverse transcriptase TEN domain' scene='Sandbox/Presentation/3' /> | <applet load='2b2a' size='400' frame='true' align='right' caption='Tetrahymena thermophila telomerase reverse transcriptase TEN domain [[2b2a]]' scene='Sandbox/Presentation/3' /> | ||
A Tetrahymena Thermophila TERT fragment consisting of residues 2-191 with an N-terminal His6 tag was cristallized in space group P3 with <scene name='Sandbox/Three/1'>three</scene> TERT molecules present in the crystallographic asymmetric unit. | A Tetrahymena Thermophila TERT fragment consisting of residues 2-191 with an N-terminal His6 tag was cristallized in space group P3 with <scene name='Sandbox/Three/1'>three</scene> TERT molecules present in the crystallographic asymmetric unit. | ||
This 23.5-kDa fragment contains only one methionine residue, making phasing by substitution with selenomethionine (SeMet) difficult. | This 23.5-kDa fragment contains only one methionine residue, making phasing by substitution with selenomethionine (SeMet) difficult. | ||
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Development of cancer chemotherapeutics directed against the reverse-transcriptase domain of telomerase has been challenging, perhaps because the enzyme needs only low activity to maintain telomeres. | Development of cancer chemotherapeutics directed against the reverse-transcriptase domain of telomerase has been challenging, perhaps because the enzyme needs only low activity to maintain telomeres. | ||
Because the TEN domain is essential for telomerase activity and its structure seems to be conserved trough evolution, this portion of TERT is a potential new target for antitelomerase compounds. | Because the TEN domain is essential for telomerase activity and its structure seems to be conserved trough evolution, this portion of TERT is a potential new target for antitelomerase compounds. | ||
==3D structures of telomerase reverse transcriptase== | |||
[[2b2a]] – TtTERT TEN domain – ''Tetrahymena thermophila''<br /> | |||
[[2r4g]] - TtTERT RNA-binding domain<br /> | |||
[[3du5]], [[3du6]] – TcTERT catalytic subunit – ''Tribolium castaneum''<br /> | |||
[[3kyl]] - TcTERT catalytic subunit + DNA + RNA | |||
=='''Additional Resources'''== | =='''Additional Resources'''== | ||