Telomerase Reverse Transcriptase: Difference between revisions
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== '''Experimental fragment with TEN''' == | == '''Experimental fragment with TEN''' == | ||
<applet load='2b2a' size='400' frame='true' align='right' caption= | <applet load='2b2a' size='400' frame='true' align='right' caption='Tetrahymena thermophila telomerase reverse transcriptase TEN domain' scene='Sandbox/Presentation/3' /> | ||
A Tetrahymena Thermophila TERT fragment consisting of residues 2-191 with an N-terminal His6 tag was cristallized in space group P3 with <scene name='Sandbox/Three/1'>three</scene> TERT molecules present in the crystallographic asymmetric unit. | A Tetrahymena Thermophila TERT fragment consisting of residues 2-191 with an N-terminal His6 tag was cristallized in space group P3 with <scene name='Sandbox/Three/1'>three</scene> TERT molecules present in the crystallographic asymmetric unit. | ||
This 23.5-kDa fragment contains only one methionine residue, making phasing by substitution with selenomethionine (SeMet) difficult. | This 23.5-kDa fragment contains only one methionine residue, making phasing by substitution with selenomethionine (SeMet) difficult. | ||