Succinyl-CoA synthetase: Difference between revisions
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==Regulation== | ==Regulation== | ||
Succinyl-CoA synthetase is not a major regulator in the Krebs cycle, making it dependent on the steps prior. However, there has been evidence that a high-affinity GDP-binding site does allosterically regulate the activity of the enzyme. It has also been seen that the binding of GDP to these allosteric sites actually leads to an increase in the rate of phosphorylation of Succinyl-Coa synthetase. This could be due to the fact that GDP is probably altering the affinity of the enzyme for GTP.<ref>Um, H.-D., and C. Klein. 1993. Evidence for allosteric regulation of succinyl-CoA synthetase. Biochem. J. 295:821–826.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1134635/]</ref> | Succinyl-CoA synthetase is not a major regulator in the Krebs cycle, making it dependent on the steps prior. However, there has been evidence that a high-affinity GDP-binding site does allosterically regulate the activity of the enzyme. It has also been seen that the binding of GDP to these allosteric sites actually leads to an increase in the rate of phosphorylation of Succinyl-Coa synthetase. This could be due to the fact that GDP is probably altering the affinity of the enzyme for GTP.<ref>Um, H.-D., and C. Klein. 1993. Evidence for allosteric regulation of succinyl-CoA synthetase. Biochem. J. 295:821–826.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1134635/]</ref> | ||
==3D structures of succinyl-CoA synthetase== | |||
===Histidine-phosphorylated succinyl-CoA synthetase=== | |||
[[1scu – EcSCS α+β subunit – ''Escherichia coli''<br /> | |||
[[2nu6]], [[2nu7]], [[2nu8]], [[2nu9]], [[2nua]] - EcSCS α (mutant) + β subunit<br /> | |||
[[1eud – pSCS α+β (mutant) subunit - pig<br /> | |||
[[2fp4]] - pSCS α+β subunit + Mg + GTP<br /> | |||
[[2fpi]], [[2fpp]] - pSCS α+β subunit | |||
===Dephosphorylated succinyl-CoA synthetase=== | |||
[[1cqi]] - EcSCS α+β subunit + Mg + ADP + PO4<br /> | |||
[[1cqj]], [[1jkj]] - EcSCS α+β subunit + PO4<br /> | |||
[[1jll]] - EcSCS α+β (mutant) subunit + PO4<br /> | |||
[[1euc – pSCS α+β (mutant) subunit + PO4<br /> | |||
[[2fpg – pSCS α+β subunit + PO4 + GDP<br /> | |||
[[1oi7 – SCS α subunit (mutant) – ''Thermos thermophilus''<br /> | |||
[[2yv1]] - SCS α subunit – ''Methanocaldococcus jannaschii''<br /> | |||
[[2yv2]] - SCS α subunit – ''Aeropyrum pernix'' | |||
==Additional Resources== | ==Additional Resources== | ||