1cvl: Difference between revisions
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[[Image:1cvl.gif|left|200px]]<br /><applet load="1cvl" size=" | [[Image:1cvl.gif|left|200px]]<br /><applet load="1cvl" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1cvl, resolution 1.6Å" /> | caption="1cvl, resolution 1.6Å" /> | ||
'''CRYSTAL STRUCTURE OF BACTERIAL LIPASE FROM CHROMOBACTERIUM VISCOSUM ATCC 6918'''<br /> | '''CRYSTAL STRUCTURE OF BACTERIAL LIPASE FROM CHROMOBACTERIUM VISCOSUM ATCC 6918'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1CVL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chromobacterium_viscosum Chromobacterium viscosum] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Known structural/functional Sites: <scene name='pdbsite=ACT:The Catalytic Triad Of Active Center Consists Of Residue ...'>ACT</scene>, <scene name='pdbsite=CA:Ca Binding Site'>CA</scene> and <scene name='pdbsite=OXY:Performed Oxyanion, Stabilized By The Amide N Atoms Of L ...'>OXY</scene>. Full crystallographic information is available from [http:// | 1CVL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chromobacterium_viscosum Chromobacterium viscosum] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Known structural/functional Sites: <scene name='pdbsite=ACT:The+Catalytic+Triad+Of+Active+Center+Consists+Of+Residue+...'>ACT</scene>, <scene name='pdbsite=CA:Ca+Binding+Site'>CA</scene> and <scene name='pdbsite=OXY:Performed+Oxyanion,+Stabilized+By+The+Amide+N+Atoms+Of+L+...'>OXY</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CVL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
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Revision as of 10:35, 3 February 2008
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CRYSTAL STRUCTURE OF BACTERIAL LIPASE FROM CHROMOBACTERIUM VISCOSUM ATCC 6918
OverviewOverview
The crystal structure of a lipase from the bacterium Chromobacterium, viscosum ATCC 6918 (CVL) has been determined by isomorphous replacement, and refined at 1.6 angstroms resolution to an R-factor of 17.8%. The, lipase has the overall topology of an alpha/beta type protein, which was, also found for previously determined lipase structures. The catalytic, triad of the active center consists of the residues Ser87, Asp263 and, His285. These residues are not exposed to the solvent, but a narrow, channel connects them with the molecular surface. This conformation is, very similar to the previously reported closed conformation of Pseudomonas, glumae lipase (PGL), but superposition of the two lipase structures, reveals several conformational differences. r.m.s. deviations greater than, 2 angstroms are found for the C alpha-atoms of the polypeptide chains from, His15 to Asp28, from Leu49 to Ser54 and from Lys128 to Gln158. Compared to, the PGL structure in the CVL structure, three alpha-helical fragments are, shorter, one beta-strand is longer and an additional antiparallel, beta-sheet is found. In contrast to PGL, CVL displays an oxyanion hole, which is stabilized by the amide nitrogen atoms of Leu17 and Gln88, and a, cis-peptide bond between Gln291 and Leu292. CVL contains a Ca2+, like the, PGL, which is coordinated by four oxygen atoms from the protein and two, water molecules.
About this StructureAbout this Structure
1CVL is a Single protein structure of sequence from Chromobacterium viscosum with as ligand. Active as Triacylglycerol lipase, with EC number 3.1.1.3 Known structural/functional Sites: , and . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a bacterial lipase from Chromobacterium viscosum ATCC 6918 refined at 1.6 angstroms resolution., Lang D, Hofmann B, Haalck L, Hecht HJ, Spener F, Schmid RD, Schomburg D, J Mol Biol. 1996 Jun 21;259(4):704-17. PMID:8683577
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