1ckn: Difference between revisions
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[[Image:1ckn.gif|left|200px]]<br /><applet load="1ckn" size=" | [[Image:1ckn.gif|left|200px]]<br /><applet load="1ckn" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ckn, resolution 2.5Å" /> | caption="1ckn, resolution 2.5Å" /> | ||
'''STRUCTURE OF GUANYLYLATED MRNA CAPPING ENZYME COMPLEXED WITH GTP'''<br /> | '''STRUCTURE OF GUANYLYLATED MRNA CAPPING ENZYME COMPLEXED WITH GTP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1CKN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1] with MN, SO4 and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50] Known structural/functional Sites: <scene name='pdbsite=GMA:Active Site LYS'>GMA</scene> and <scene name='pdbsite=GMB:Active Site LYS'>GMB</scene>. Full crystallographic information is available from [http:// | 1CKN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50] Known structural/functional Sites: <scene name='pdbsite=GMA:Active+Site+LYS'>GMA</scene> and <scene name='pdbsite=GMB:Active+Site+LYS'>GMB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CKN OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: nucleotidyltransferase]] | [[Category: nucleotidyltransferase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:34:43 2008'' | ||
Revision as of 10:34, 3 February 2008
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STRUCTURE OF GUANYLYLATED MRNA CAPPING ENZYME COMPLEXED WITH GTP
OverviewOverview
We have solved the crystal structure of an mRNA capping enzyme at 2.5 A, resolution. The enzyme comprises two domains with a deep, but narrow, cleft between them. The two molecules in the crystallographic asymmetric, unit adopt very different conformations; both contain a bound GTP, but one, protein molecule is in an open conformation while the other is in a closed, conformation. Only in the closed conformation is the enzyme able to bind, manganese ions and undergo catalysis within the crystals to yield the, covalent guanylated enzyme intermediate. These structures provide direct, evidence for a mechanism that involves a significant conformational change, in the enzyme during catalysis.
About this StructureAbout this Structure
1CKN is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1 with , and as ligands. Active as mRNA guanylyltransferase, with EC number 2.7.7.50 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes., Hakansson K, Doherty AJ, Shuman S, Wigley DB, Cell. 1997 May 16;89(4):545-53. PMID:9160746
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