1bws: Difference between revisions
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[[Image:1bws.jpg|left|200px]]<br /><applet load="1bws" size=" | [[Image:1bws.jpg|left|200px]]<br /><applet load="1bws" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1bws, resolution 2.2Å" /> | caption="1bws, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE OF GDP-4-KETO-6-DEOXY-D-MANNOSE EPIMERASE/REDUCTASE FROM ESCHERICHIA COLI A KEY ENZYME IN THE BIOSYNTHESIS OF GDP-L-FUCOSE'''<br /> | '''CRYSTAL STRUCTURE OF GDP-4-KETO-6-DEOXY-D-MANNOSE EPIMERASE/REDUCTASE FROM ESCHERICHIA COLI A KEY ENZYME IN THE BIOSYNTHESIS OF GDP-L-FUCOSE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1BWS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NDP as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=CAT:Catalytic Residues'>CAT</scene>. Full crystallographic information is available from [http:// | 1BWS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=CAT:Catalytic+Residues'>CAT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWS OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: gdp-l-fucose biosynthesis]] | [[Category: gdp-l-fucose biosynthesis]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:34:09 2008'' | ||
Revision as of 10:34, 3 February 2008
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CRYSTAL STRUCTURE OF GDP-4-KETO-6-DEOXY-D-MANNOSE EPIMERASE/REDUCTASE FROM ESCHERICHIA COLI A KEY ENZYME IN THE BIOSYNTHESIS OF GDP-L-FUCOSE
OverviewOverview
BACKGROUND: The process of guanosine 5'-diphosphate L-fucose, (GDP-L-fucose) biosynthesis is conserved throughout evolution from, prokaryotes to man. In animals, GDP-L-fucose is the substrate of, fucosyltransferases that participate in the biosynthesis and remodeling of, glycoconjugates, including ABH blood group and Lewis-system antigens. The, 'de novo' pathway of GDP-L-fucose biosynthesis from GDP-D-mannose involves, a GDP-D-mannose 4,6 dehydratase (GMD) and a GDP-4-keto-6-deoxy-D-mannose, epimerase/reductase (GMER). Neither of the catalytic mechanisms nor the, three-dimensional structures of the two enzymes has been elucidated yet., The severe leukocyte adhesion deficiency (LAD) type II genetic syndrome is, known to result from deficiencies in this de novo pathway. RESULTS: The, crystal structures of apo- and holo-GMER have been determined at 2.1 A and, 2.2 A resolution, respectively. Each subunit of the homodimeric (2 x 34, kDa) enzyme is composed of two domains. The N-terminal domain, a, six-stranded Rossmann fold, binds NADP+; the C-terminal domain (about 100, residues) displays an alpha/beta topology. NADP+ interacts with residues, Arg12 and Arg36 at the adenylic ribose phosphate; moreover, a protein loop, based on the Gly-X-X-Gly-X-X-Gly motif (where X is any amino acid), stabilizes binding of the coenzyme diphosphate bridge. The nicotinamide, and the connected ribose ring are located close to residues Ser107, Tyr136, and Lys140, the putative GMER active-site center. CONCLUSIONS: The GMER, fold is reminiscent of that observed for UDP-galactose epimerase (UGE), from Escherichia coli. Consideration of the enzyme fold and of its main, structural features allows assignment of GMER to the, reductase-epimerase-dehydrogenase (RED) enzyme homology superfamily, to, which short-chain dehydrogenase/reductases (SDRs) also belong. The, location of the NADP+ nicotinamide ring at an interdomain cleft is, compatible with substrate binding in the C-terminal domain.
About this StructureAbout this Structure
1BWS is a Single protein structure of sequence from Escherichia coli with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
GDP-4-keto-6-deoxy-D-mannose epimerase/reductase from Escherichia coli, a key enzyme in the biosynthesis of GDP-L-fucose, displays the structural characteristics of the RED protein homology superfamily., Rizzi M, Tonetti M, Vigevani P, Sturla L, Bisso A, Flora AD, Bordo D, Bolognesi M, Structure. 1998 Nov 15;6(11):1453-65. PMID:9817848
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