1bhn: Difference between revisions
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[[Image:1bhn.gif|left|200px]]<br /><applet load="1bhn" size=" | [[Image:1bhn.gif|left|200px]]<br /><applet load="1bhn" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1bhn, resolution 2.4Å" /> | caption="1bhn, resolution 2.4Å" /> | ||
'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA'''<br /> | '''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1BHN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with 35G as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Known structural/functional Sites: <scene name='pdbsite=A:C-Gmp And Gdp Are Found (Each w. Half Occupancy) In The ...'>A</scene>, <scene name='pdbsite=AND F:C-Gmp And Gdp Are Found (Each w. Half Occupancy) In The ...'>AND F</scene>, <scene name='pdbsite=B:C-Gmp And Gdp Are Found (Each w. Half Occupancy) In The ...'>B</scene>, <scene name='pdbsite=C:C-Gmp And Gdp Are Found (Each w. Half Occupancy) In The ...'>C</scene>, <scene name='pdbsite=D:C-Gmp And Gdp Are Found (Each w. Half Occupancy) In The ...'>D</scene> and <scene name='pdbsite=E:C-Gmp And Gdp Are Found (Each w. Half Occupancy) In The ...'>E</scene>. Full crystallographic information is available from [http:// | 1BHN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=35G:'>35G</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Known structural/functional Sites: <scene name='pdbsite=A:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>A</scene>, <scene name='pdbsite=AND F:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>AND F</scene>, <scene name='pdbsite=B:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>B</scene>, <scene name='pdbsite=C:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>C</scene>, <scene name='pdbsite=D:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>D</scene> and <scene name='pdbsite=E:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>E</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHN OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:32:56 2008'' | ||
Revision as of 10:32, 3 February 2008
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NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA
OverviewOverview
The crystal structures of two isoforms of nucleoside diphosphate kinase, from bovine retina overexpressed in Escherischia coli have been determined, to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and, the fold of the monomer is in agreement with NDP-kinase structures from, other biological sources. Although the polypeptide chains of the two, isoforms differ by only two residues, they crystallize in different space, groups. NBR-A crystallizes in space group P212121 with an entire hexamer, in the asymmetric unit, while NBR-B crystallizes in space group P43212, with a trimer in the asymmetric unit. The highly conserved, nucleotide-binding site observed in other nucleoside diphosphate kinase, structures is also observed here. Both NBR-A and NBR-B were crystallized, in the presence of cGMP. The nucleotide is bound with the base in the anti, conformation. The NBR-A active site contained both cGMP and GDP each bound, at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the, purification process. The NBR-B active site contained only cGMP.
About this StructureAbout this Structure
1BHN is a Single protein structure of sequence from Bos taurus with as ligand. Active as Nucleoside-diphosphate kinase, with EC number 2.7.4.6 Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.
ReferenceReference
The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina., Ladner JE, Abdulaev NG, Kakuev DL, Tordova M, Ridge KD, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1127-35. PMID:10329774
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