1as6: Difference between revisions
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[[Image:1as6.jpg|left|200px]]<br /><applet load="1as6" size=" | [[Image:1as6.jpg|left|200px]]<br /><applet load="1as6" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1as6, resolution 1.8Å" /> | caption="1as6, resolution 1.8Å" /> | ||
'''STRUCTURE OF NITRITE BOUND TO OXIDIZED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE'''<br /> | '''STRUCTURE OF NITRITE BOUND TO OXIDIZED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1AS6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with CU and NO2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.3 1.7.99.3] Known structural/functional Sites: <scene name='pdbsite=CU1:Cu Site'>CU1</scene>, <scene name='pdbsite=CU2:Cu Site'>CU2</scene>, <scene name='pdbsite=CU3:Cu Site'>CU3</scene>, <scene name='pdbsite=CU4:Cu Site'>CU4</scene>, <scene name='pdbsite=CU5:Cu Site'>CU5</scene> and <scene name='pdbsite=CU6:Cu Site'>CU6</scene>. Full crystallographic information is available from [http:// | 1AS6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=NO2:'>NO2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.3 1.7.99.3] Known structural/functional Sites: <scene name='pdbsite=CU1:Cu+Site'>CU1</scene>, <scene name='pdbsite=CU2:Cu+Site'>CU2</scene>, <scene name='pdbsite=CU3:Cu+Site'>CU3</scene>, <scene name='pdbsite=CU4:Cu+Site'>CU4</scene>, <scene name='pdbsite=CU5:Cu+Site'>CU5</scene> and <scene name='pdbsite=CU6:Cu+Site'>CU6</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AS6 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
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Revision as of 10:31, 3 February 2008
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STRUCTURE OF NITRITE BOUND TO OXIDIZED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE
OverviewOverview
The structures of oxidized, reduced, nitrite-soaked oxidized and, nitrite-soaked reduced nitrite reductase from Alcaligenes faecalis have, been determined at 1.8-2.0 A resolution using data collected at -160, degrees C. The active site at cryogenic temperature, as at room, temperature, contains a tetrahedral type II copper site liganded by three, histidines and a water molecule. The solvent site is empty when crystals, are reduced with ascorbate. A fully occupied oxygen-coordinate nitrite, occupies the solvent site in crystals soaked in nitrite. Ascorbate-reduced, crystals soaked in a glycerol-methanol solution and nitrite at -40 degrees, C remain colorless at -160 degrees C but turn amber-brown when warmed, suggesting that NO is released. Nitrite is found at one-half occupancy., Five new solvent sites in the oxidized nitrite bound form exhibit defined, but different occupancies in the other three forms. These results support, a previously proposed mechanism by which nitrite is bound primarily by a, single oxygen atom that is protonable, and after reduction and cleavage of, that N-O bond, NO is released leaving the oxygen atom bound to the Cu site, as hydroxide or water.
About this StructureAbout this Structure
1AS6 is a Single protein structure of sequence from Alcaligenes faecalis with and as ligands. Active as Transferred entry: 1.7.2.1, with EC number 1.7.99.3 Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.
ReferenceReference
Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic implications., Murphy ME, Turley S, Adman ET, J Biol Chem. 1997 Nov 7;272(45):28455-60. PMID:9353305
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